ChromograninCatalogue number: CH500
LK2H10 antibody to chromogranin stains both, hormone secreting cells (except for neurohypophysis) and all tumours derived from endocrine glands. Less intense staining is usually found in small cell lung carcinoma (SCLC), Merkel cell carcinoma and Neuroblastoma. Chromogranin is a marker of the diffuse neuroendocrine system, since it is a constituent of most secretory granula of endocrine cells It was detected as the main component of chromaffin cells in the adrenal medulla. Chromogranin A and other Chromogranins.
Immunogen: Human Phaeochromocytoma (solid tissue)
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Species Reactivity: Human, monkey (cattle, pig)
IHC (C, P)
Incubation Time: 60 min at RT
Working Concentration: (RTU) neat
Pre-Treatment: No protease pre-treatment necessary
Positive Control: Adrenal medulla, anterior pituitary, pancreatic islets
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
1. Lloyd R.V. and Wilson B.S. (1983) Specific endocrine tissue marker defined by a monoclonal antibody. Science; 222, 628 ff. 2. Wilson B.S. and Lloyd .V. (1984) Detection of chromogranin in neuroendocrine cells with a monoclonal antibody. Am. J. Pathol. 115; 458 ff. 3. DeStephano D.B., Lloyd R.V., Pike A.N., and Wilson B.S. (1984) Pituritary adenomas: An immunohistochemical study of hormone production and chromogranin localization. Am. J. Pathol. 116; 464 ff. 4. Wilson B.S., Phan S.H., Lloyd R.V. (1986) Chromogranin from normal human adrenal glands: Purification by monoclonal antibody affinity chromatography and partial N-terminal amino acid sequence. Reggul. Pept. 13; 207 ff. 5. Hawkins KL, Lloyd RV, Toy KA (1989) Immunohistochemical localization of chromogranin A in normal tissues from laboratory animals. Vet Pathol. Nov;26(6):488-98.