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Chromogranin


Catalogue number: CH500

CloneLK2H10
IsotypeIgG1,k
Units5 ml
HostMouse
Application IHC (C
P)
Background
LK2H10 antibody to chromogranin stains both, hormone secreting cells (except for neurohypophysis) and all tumours derived from endocrine glands. Less intense staining is usually found in small cell lung carcinoma (SCLC), Merkel cell carcinoma and Neuroblastoma. Chromogranin is a marker of the diffuse neuroendocrine system, since it is a constituent of most secretory granula of endocrine cells It was detected as the main component of chromaffin cells in the adrenal medulla. Chromogranin A and other Chromogranins.

Positive Control
Adrenal medulla, anterior pituitary, pancreatic islets

Pre-Treatment
No protease pre-treatment necessary

Immunogen
Human Phaeochromocytoma (solid tissue)

Purification Method
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

Working Concentration
(RTU) neat

Incubation Time
60 min at RT

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

Applications
IHC (C, P)

Cross Reactivity
Human, monkey (cattle, pig)

Storage
2-8°C

References
1. Lloyd R.V. and Wilson B.S. (1983) Specific endocrine tissue marker defined by a monoclonal antibody. Science; 222, 628 ff. 2. Wilson B.S. and Lloyd .V. (1984) Detection of chromogranin in neuroendocrine cells with a monoclonal antibody. Am. J. Pathol. 115; 458 ff. 3. DeStephano D.B., Lloyd R.V., Pike A.N., and Wilson B.S. (1984) Pituritary adenomas: An immunohistochemical study of hormone production and chromogranin localization. Am. J. Pathol. 116; 464 ff. 4. Wilson B.S., Phan S.H., Lloyd R.V. (1986) Chromogranin from normal human adrenal glands: Purification by monoclonal antibody affinity chromatography and partial N-terminal amino acid sequence. Reggul. Pept. 13; 207 ff. 5. Hawkins KL, Lloyd RV, Toy KA (1989) Immunohistochemical localization of chromogranin A in normal tissues from laboratory animals. Vet Pathol. Nov;26(6):488-98.

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

Chromogranin

$158.00

SKU: CH500 Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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