Chicken anti Green Fluorescent Protein (GFP) [Neisseria gonorrhoeae]Catalogue number: AGFP
|Product Type||Primary Antibodies|
Chickens were immunized with recombinant full-length bacterial gonococci Green Fluorescent Protein (GFP) [Neisseria gonorrhoeae] expressed in E. coli cells. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified.
Product Form: Egg yolk-derived purified IgY
Formulation: Phosphate buffered saline‚ pH 7.2 with 0.075% sodium azide
Purification Method: Egg yolk-derived purified IgY (non affinity purified)
Concentration: See vial for concentration.
In a Western blot, the anti-GFP used at between 1:3000 and 1:5000 dilution could detect 10 ng of GFP in an A549 cell lysate (see image). Optimal conjugate working dilutions should be determined for your particular assay conditions.
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles.
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Safety Datasheet(s) for this product:
Chicken anti Green Fluorescent Protein (GFP) [Neisseria gonorrhoeae]