Catalogue

Background
Glyphosphate is one of the most used herbicides. It is contained in Roundup. Due to its watersolubility it can be found in the water environment.

Source
Chicken egg yolk

Immunogen: BSA-Glyphosate conjugate: glyphosate conjugated to BSA via the free carboxylic acid and amine coupling (EDC/NHS) protocol.

Product
Each vial contains 1 ml 5mg/ml of purified polyclonal IgY antibody in PBS pH 7.4 containing 0.02% sodium azide.

Purification Method: PEG precipitation

Specificity
Target: Glyphosate, CAS no.: 1071-83-6, Solubility: Water.

Applications
Antibody can be used in ELISA and coupled to column-material for IAC. Depending on conjugate: dilution 2,000 from the delivered solution. (The titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested with ELISA). The antibody is applicable for any type of (waste) water sample. The detection limit is 2.5 µg/l, while the end concentration is 100 µg/l. The standard curve runs from 5 – 100 µg/l. For an optimal ELISA result it is crucial to use Greiner high affinity plates; As assaybuffer 0.1M PBS, 1% BSA heat-treated, pH 7.4 is recommended.

Storage
Store at 4 °C or aliquot and store at -20 °C for longer periods. Precautions should be taken upon storage for longer periods. Problems of long term stability may occur with highly diluted solutions. For long term storage purposes in solution the addition of sodium azide at 0.02 % is advised with the appropriate precautions of use.

Shipping Conditions: At ambient temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.