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Chicken anti Clostridium difficile Toxin A

Catalogue number: ACdTA

Product Type Primary Antibodies
Units0.1 mg
Application ELISA
Western Blotting

Chickens were immunized with highly purified Toxin A purified from Clostridium difficile. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Clostridium difficile Toxin A was immunoaffinity purified using the immunogen immobilized on a solid support.


Product Form: Affinity purified

Formulation: Phosphate buffered saline‚ pH 7.2 with 0.075% sodium azide

Purification Method: Egg-yolk derived IgY, affinity purified.

Concentration: See vial for concentration.

This antibody is suitable for ELISA and Western Blotting procedures. By Western blot, a 1:4000 dilution of the IgY was an effective probe for 1 ug of Toxin A. By Direct ELISA, the half-point titre was 1:20,000 using a 2 ug/ml coating of Toxin A. Optimal working dilutions should be determined for your particular assay conditions.

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles.

Shipping Conditions: Ship at ambient temperature.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

1. Bochner, B and X. Lei. 2015. Compositions and Methods to Inactivate and/or Reduce Production of Microbial Toxins. United States Patent Application 2015 0374786.
2. Tussey, L., Song, L. and S. Umlauf. 2016. Clostridium difficile Compositions and Methods of Use. United States Patent Application 2016016667.

Safety Datasheet(s) for this product:
EA_Sodium Azide
/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf

Chicken anti Clostridium difficile Toxin A


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