Chicken anti Clostridium difficile Binary Toxin, Subunit ACatalogue number: ACdBTSA
|Product Type||Primary Antibodies|
Chickens were immunized with highly purified Binary Toxin, A subunit, purified from Clostridium difficile. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Clostridium difficile Binary Toxin, Subunit A was immunoaffinity purified using the immunogen immobilized on a solid support.
Product Form: Affinity purified
Formulation: Phosphate buffered saline‚ pH 7.2 with 0.075% sodium azide
Purification Method: Egg-yolk derived IgY Affinity Purified
Concentration: See vial for concentration.
This antibody has been tested in direct ELISA and Western blot. A 1:4000 dilution was an effective probe for 125 ng of C. difficile Binary Toxin A Subunit. The antibody titre, measured at the midpoint, was 1:56,000 of a 2.0 mg/ml solution (~90 ng/ml) using a 2 ug coating of C. difficile Binary Toxin, A Subunit. Optimal working dilutions should be determined for your particular assay conditions.
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles.
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Safety Datasheet(s) for this product:
Chicken anti Clostridium difficile Binary Toxin, Subunit A