Chicken anti C-MYC Epitope TagCatalogue number: ACMYC
|Product Type||Primary Antibodies|
Chickens were immunized with EQKLISEEDL (C-MYC) conjugated to KLH. After multiple immunizations in Freunds adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.
Product Form: Affinity purified
Formulation: Phosphate buffered saline, pH 7.2 containing 0.1% sodium azide
Concentration: See vial for concentration.
ELISA: coating: 1:50 – 1:200; probing: 1:5000 - 1:20,000Western Blots: 1:5000 dilution. Flow Cytometry: 1:100 - 1:300 dilution (see embedded figures).
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles.
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
1. Mata-Fink J, Kriegsman B, Yu HX, Zhu H, Hanson MC, Irvine DJ and KD Wittrup. 2013. Rapid Conformational Epitope Mapping of Anti-gp120 Antibodies with a Designed Mutant Panel Displayed on Yeast. J Mol Biol. 425:444-56. 2. Van Deventer JA, Wittrup KD. 2014.Yeast surface display for antibody isolation: library construction, library screening, and affinity maturation. Methods Mol Biol. 1131:151-81. 3. Wang, S., Mata-Fink J., Kriegsman B, Hanson, M, Irvien DJ, Eisen HN, Burtn DR, Wittrup, KD, Kardar M, Chakraborty, AK. 2015. Manipulating the Selection Forces during Affinity Maturation to Generate Cross-Reactive HIV Antibodies.Cell 160(4):785-97. 4. Heimer, BW, Tam, BE and HD Sikes. 2015. Characterization and directed evolution of a methyl-binding protein for high-sensitivity DNA methylation analysis. Protein Eng Des Sel 28:543-551. 5. de Picciotto S, Dickson PM, Traxlmayr MW, Marques BS, Socher E, Zhao S1, Cheung S, Kiefer JD, Wand AJ, Griffith LG, Imperiali B, Wittrup KD. 2016. Design Principles for SuCESsFul Biosensors: Specific Fluorophore/Analyte Binding and Minimization of Fluorophore/Scaffold Interactions.J Mol Biol. 428(20):4228-4241. 6. Traxlmayr MW, Kiefer JD, Srinivas RR, Lobner E, Tisdale AW, Mehta NK, Yang NJ, Tidor B, Wittrup KD. 2016. Strong Enrichment of Aromatic Residues in Binding Sites from a Charge-neutralized Hyperthermostable Sso7d Scaffold Library. J Biol Chem 291 (43): 22496-22508. 7. Kauke, MJ, MW, Traxlmayr, MW, Parker, JA, Kiefer, JD, Knihtila, R, McGee, J, Verdine, G, Mattos, C and KD Wittrup. 2017. An engineered protein antagonist of K-Ras/B-Raf interaction. Sci. Rep. 7: 5831. 8. Angelini A, Miyabe Y, Newsted D, Kwan BH, Miyabe C, Kelly RL, Jamy MN, Luster AD, Wittrup KD. Directed evolution of broadly crossreactive chemokine-blocking antibodies efficacious in arthritis. 2018. Nat Commun. 13;9(1):1461. doi: 10.1038/s41467-018-03687-x
2. -Karlsson,A, Protein and peptide engineering for improving therapies for applications in Human health.
Safety Datasheet(s) for this product:
Chicken anti C-MYC Epitope Tag