Chicken anti Botulinum Neurotoxin Type BCatalogue number: AHcB
|Product Type||Primary Antibodies|
Chickens were immunized with highly purified Botulinum Neurotoxin Type B (Clostridium botulinum). After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Botulinum Neurotoxin Type B was immunoaffinity purified using the immunogen immobilized on a solid support.
Product Form: Affinity purified
Formulation: Phosphate buffered saline‚ pH 7.2 with 0.075% sodium azide
Purification Method: Egg-yolk derived IgY Affinity Purified
Concentration: See vial for concentration.
This antibody is suitable for ELISA and Western Blotting procedures. For direct ELISA, a 1:66,000 dilution detects Botulinum HccB and a 1:42,000 dilutions detects Clostridium botulinum Type B (BTB). For Western blot, the recommended dilution is between 1:1000 and 1:2000. Optimal working dilutions should be determined for your particular assay conditions.
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles.
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
1. Zhang Y., Lou J., Jenko K.L., Marks, J.D. and S.M. Varnum. 2012. Simultaneous and sensitive detection of six serotypes of botulinum neurotoxin using enzyme-linked immunosorbent assay-based protein antibody microarrays. Anal. Biochem. 1430(2):185-92.
Safety Datasheet(s) for this product:
Chicken anti Botulinum Neurotoxin Type B