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Chicken anti Botulinum Neurotoxin Type B


Catalogue number: AHcB

Product Type Primary Antibodies
Units0.1 mg
HostChicken
Product Form
Affinity purified

Concentration
See vial for concentration.

Formulation
Phosphate buffered saline‚ pH 7.2 with 0.075% sodium azide

Source
Chickens were immunized with highly purified Botulinum Neurotoxin Type B (Clostridium botulinum). After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Botulinum Neurotoxin Type B was immunoaffinity purified using the immunogen immobilized on a solid support.

Product
Liquid; 0.05 mL

Applications
This antibody is suitable for ELISA and Western Blotting procedures. For direct ELISA, a 1:66,000 dilution detects Botulinum HccB and a 1:42,000 dilutions detects Clostridium botulinum Type B (BTB). For Western blot, the recommended dilution is between 1:1000 and 1:2000. Optimal working dilutions should be determined for your particular assay conditions.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles.

References
1. Zhang Y., Lou J., Jenko K.L., Marks, J.D. and S.M. Varnum. 2012. Simultaneous and sensitive detection of six serotypes of botulinum neurotoxin using enzyme-linked immunosorbent assay-based protein antibody microarrays. Anal. Biochem. 1430(2):185-92.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. 

Safety Datasheet(s) for this product:

Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Chicken anti Botulinum Neurotoxin Type B

$242.00

SKU: AHcB Categories: ,