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Chicken anti Botulinum Neurotoxin Type B

Catalogue number: AHcB

Product Type Primary Antibodies
Units0.1 mg
Application ELISA
Western Blotting

Chickens were immunized with highly purified Botulinum Neurotoxin Type B (Clostridium botulinum). After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Botulinum Neurotoxin Type B was immunoaffinity purified using the immunogen immobilized on a solid support.


Product Form: Affinity purified

Formulation: Phosphate buffered saline‚ pH 7.2 with 0.075% sodium azide

Purification Method: Egg-yolk derived IgY Affinity Purified

Concentration: See vial for concentration.

This antibody is suitable for ELISA and Western Blotting procedures. For direct ELISA, a 1:66,000 dilution detects Botulinum HccB and a 1:42,000 dilutions detects Clostridium botulinum Type B (BTB). For Western blot, the recommended dilution is between 1:1000 and 1:2000. Optimal working dilutions should be determined for your particular assay conditions.

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

1. Zhang Y., Lou J., Jenko K.L., Marks, J.D. and S.M. Varnum. 2012. Simultaneous and sensitive detection of six serotypes of botulinum neurotoxin using enzyme-linked immunosorbent assay-based protein antibody microarrays. Anal. Biochem. 1430(2):185-92.

Safety Datasheet(s) for this product:
EA_Sodium Azide
/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf

Chicken anti Botulinum Neurotoxin Type B


SKU: AHcB Categories: ,