CEA, Carcinoembryonic Antigen, CD66eCatalogue number: CD66E2-1
CEA is frequently used as a differential marker for adenocarcinoma and mesothelioma. Carcinoembryonal Antigen (CEA) is a high molecular glycoprotein (180 kD), which is detected in foetal colon and in colorectal carcinoma. It is a component of the s. c. glycocalyx of the embryonal endodermal epithelium. In normal adult colon it is found in very small amount only. In colorectal carcinoma and metastases of intestinal carcinoma however, it is strongly exprimed. Human CEA.
Immunogen: Colon carcinoma extract from human tissue
Purified antibody in PBS pH 7.3, contains BSA and sodium azide (0,09%)
Purification Method: Purified antibody in PBS pH 7.3, contains BSA and sodium azide (0,09%)
Concentration: 50 µg/ml
Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Species Reactivity: Human
Incubation Time: 60 min at RT
Working Concentration: (liquid conc.)
Pre-Treatment: Improvement of staining with Unmasking Fluid G (Art. No. DE007) or Unmasking Fluid C (Art. No. DE000)
Positive Control: Colon carcinoma
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
1. Sheahan K., O'Brien M.J., Burke B., Dervan P.A., O'Keane J.C., Gottlieb L.S. et al. (1990) Differential reactivities of carcinoembryonic antigen (CEA) and CEA-related monoclonal and polyclonal antibodies in common epithelial malignancies. Am. J. Clin. Pathol. 94; 157-164. . 2. Ghosh A.K., Gatter K.C., Dunnill M.S., Mason D.Y. (1987) Immunohistochemical staining of reactive Mesothelium, mesothelioma, and Lung carcinoma with a panel of monoclonal antibodies. J. Clin. Pathol. 40; 19-25. 3. Nap M., Hammarström M.-L., Börmer O., Hammarström S., Wagener C., Handt S., et al. (1992) Cancer Res. 52; 23-39
Database Name: UniProt
Accession number: P06731
CEA, Carcinoembryonic Antigen, CD66e