CD68 (PG-M1) Macrophages, humanCatalogue number: CD68-5
The antibody PG-M1 is well suited for the detection of tissue macrophages including Kupffer cells and spleen macrophages. Among a number of CD68 antibodies PG-M1 is one of the most specific ones, since it stains neither myeloid precursor cells, nor granulocytes or dendritic cells. In acute myeloid leukaemia (AML) types M4 and M5 will be stained, while M1 - M3, M6 and M7 remain unstained. CD68 is a transmembrane antigen, which is localised mainly in lysosomes. It belongs to the family of LAMP glycoproteins, which are involved in lysosomal transport and endocytosis respectively. The exact function of the CD68 molecule is not known. CD68 transmembran glycoprotein MW 110 kDa, localised in macrophages.
Immunogen: Gaucher cells
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Species Reactivity: Human
Incubation Time: 60 min at RT
Working Concentration: (RTU) neat
Pre-Treatment: Heat pre-treatment of formalin-fixed tissue with Unmasking Fluid C, E or G (Art. No. DE000, DE006, and DE007). Unmasking with Pronase (Art. No. DE110) or Proteinase K is also suitable.
Positive Control: Tonsil, spleen
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
1. Fukuda M. (1991) Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficing. J. Biol. Chem. 266; 21327-21330. 2. Holness C.I. and Simmons D.L. (1993) Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins. Blood 81; 1607-1613. 3. Falini B. Flenghi L., Pileri S., Gambacorta M., Bigerna B., Durkop H., et al. (1993) PG-M1: a new monoclonal antibody directed against a fixative resistant epitope on the macrophage-restricted form of the CD68 molecule. Am. J. Pathol. 142; 1359-1372. 4. Falini B. Flenghi L., Pileri S., Stein H., Dürkop H., Pasqualucci L., et al. M15.1 PG-M1, a new mAb directed against a fixative-resitant, macrophage-restricted epitope of the CD68 molecule. In: Schlossman et al.eds. Leucocyte Typing V. White Cell Differentiation Antigens. Oxford University Press 1995 pp 928-930.
CD68 (PG-M1) Macrophages, human