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CD68 (KP1) Macrophages, human


Catalogue number: CD68KP1-0.5

CloneKP1
IsotypeIgG1, k
Units0.5 ml
HostMouse
Application IHC(C
P)
Background
The antibody KP1 is well suited for the detection of tissue macrophages including Kupffer cells and spleen macrophages. CD68 is expressed on macrophages and monocytes. KP-1 is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. KP-1 reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Tumors of lymphoid origin are usually not stained. CD68 transmembran glycoprotein MW 110 kDa, localised in macrophages.

Positive Control
Tonsil, spleen, lymph node

Pre-Treatment
Unmasking with Pronase (Art. No. DE110)

Immunogen
Subcellular fraction of human alveolar macrophages

Purification Method
Purified antibody with 0.2% BSA and 15 mM sodium azide as preservative

Working Concentration
(liquid conc.) 1:100-1:200

Incubation Time
60 min at RT

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Product
Purified antibody with 0.2% BSA and 15 mM sodium azide as preservative

Applications
IHC(C, P)

Cross Reactivity
Human, monkey, cat and rat. Does not react with pig, dog and chicken

Storage
2-8°C

References
1. Fukuda M. (1991) Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficing. J. Biol. Chem. 266; 21327-21330. 2. Holness C.I. and Simmons D.L. (1993) Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins. Blood 81; 1607-1613. 3. Pulford KAF, Rigney EM, Micklem KJ, Jones M, Stross WP, Gatter KC, et al. (1989) KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections. J Clin Pathol;42:414-21. 4. Warnke RA, Pulford KAF, Pallesen G, Ralfkiaer E, Brown DC, Gatter KC, et al. (1989) Diagnosis of myelomonocytic and macrophage neoplasms in routinely processed tissue biopsies with monoclonal antibody KP1. Am J Pathol;135:1089-95.

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

CD68 (KP1) Macrophages, human

$474.00

SKU: CD68KP1-0.5 Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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