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CD57, NK Cells


Catalogue number: CD57-1

CloneNK-1
IsotypeIgM
Units1 ml
HostMouse
Application IHC(C
P)
Background
CD57 (NHK-1) is a glycoprotein with an oligosaccharide antigen determinant, which is localized especially on NK-cells, but also on subsets of T- and B-cells. T-cells expriming CD57 are upregulated in patients with HIV infection and after transplantation. The antigen is also exprimed by neuronal tissues and most neuroendocrine cells. human CD57, a 110 kDa membrane protein of human lymphocytes.

Positive Control
Brain, adrenal medulla

Pre-Treatment
No pre-treatment necessary

Immunogen
Preparation of a HSB-2 T-cell line

Purification Method
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Working Concentration
(liquid conc.) 1:10-1:100

Incubation Time
60 min at RT

Secondary Reagents
As secondary reagents we recommend the use of biotinylated anti-mouse IgM antibody (Art. No. BA-2020) in combination with streptavidin conjugates or ABC systems.

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Applications
IHC(C, P)

Cross Reactivity
Human

Storage
2-8°C

References
1. Toru Abo, et al. (1984) A differentiation antigen of human NK and K cells, identified by a monoclonal antibody (NHK-1) J. Immunol. 127: 1024-1029.

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

CD57, NK Cells

$553.00

SKU: CD57-1 Categories: ,