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CD54, ICAM-1


Catalogue number: CD54-1

Clone1304
IsotypeIgG2a
Units100 µg
HostMouse
Application IHC(C
P)
Background
Monoclonal antibody 1304 detects CD54 (ICAM-1) and is useful for the detection of activated endothelial cells and other activated haematopoietic cells. The antibody inhibits the binding of natural killer (NK) cells to target cells (K562). Intercellular adhesion molecule (ICAM) 1 or CD54 was initially identified with monoclonal antibody RR1/1 as a widely distributed, cytokine-inducible counter-receptor for lymphocyte-function-associated antigen (LFA) 1 (CD11a/CD18). Granulomas (macrophages/epitheloid cells) in inflamed colon from Crohn's disease and follicular dendritic cells in the tonsil from chronic tonsillitis are strongly positive for CD54 antigen. Also, CD54 is found on activated CD56+/CD3- NK cells. Human CD54, the antibody detects an epitope in domain 1 of the 95 kDa transmembrane protein..

Positive Control
Human Tonsil

Pre-Treatment
Formaldehyde-fixed tissue slices are preferably pre-treated with proteinase K.

Immunogen
Cultivated human endothelial cells

Purification Method
Affinity purified, lyophilized antibody. Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.2mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 10mg/ml bovine serum albumin (BSA) as a stabilizer and 0.01% thimerosal as a preservative.

Working Concentration
(lyophilisate) 2 µg/ml (IHC-P)

Concentration
0.1 mg lyophilized

Incubation Time
60 min at RT or 18 hr at 2-8°C

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Product
Affinity purified, lyophilized antibody. Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.2mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 10mg/ml bovine serum albumin (BSA) as a stabilizer and 0.01% thimerosal as a preservative.

Applications
IHC(C, P)

Cross Reactivity
Human

Storage
2-8°C

References
1. Albelda S.M., and Buck C.A. (1990) Integrins and other cell adhesion molecules. FASEB J. 4; 2868-2880. 2. Freudenthal P.S., and Steinmann R.M. (1990) The distinct surface of human blood dendritic cells, as observed after an improved isolation method. PNAS 87; 7698-7702. 3. Klickstein L.B., and Springer T.A. (1995) CD54 (ICAM-1)cluster report. In Leucocyte Typing V: White Cell Differentiation Antigens. S. Schlossman et al. eds.Oxford University Press pp.1548-1550.

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

CD54, ICAM-1

$648.00

SKU: CD54-1 Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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