CD34, humanCatalogue number: CD34-5
The antibody clone QBEND/10 reacts with an epitope on endothelial cells and haematopoietic progenitor cells, which is not sensitive to neuraminidase but to chymopapain and glycoprotease (CD34 class II antibodies). It may be used for the detection of endothelial cells and haematopoietic progenitor cells in tissues, cell smears and cell cultures. The CD34 molecule is a glycoprotein (110 kDa), which is expressed as well by endothelial cells as by haematopoietic precursor cells (BFU-E, CFU-GM, CFU-Meg, CFU-Eo, CFU-osteoclasts and CFU-blasts) in human bone marrow. CD34 is the earliest marker of colony building cells in bone marrow. The molecule is heavily glycosylated in the N-terminal extracellular region and therefore sensitive to neuraminidase (target of CD34 class I antibodies). This region is followed by a globular Cys-rich domain. The cytoplasmic portion of the molecule contains sites for proteinkinase C and tyrosine phosphorylation. Human CD34 antigen (Epitop neuraminidase resistant , glycoprotease- and chymopapain-sensitive).
Immunogen: Purified human endothelial vesicles
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Species Reactivity: Human, Rhesus Monkey, no cross-reactivity with cattle, dog, rat and sheep
Incubation Time: 60 min at RT
Working Concentration: (RTU) neat
Pre-Treatment: No protease pre-treatment!, Pre-treatment with unmasking fluid G (Art. No. DE007) or unmasking fluid C (Art. No. DE000) at 90-100°C may be helpful.
Positive Control: Tonsil, bone marrow
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
1. Civin C.I., Strauss L.C., Brovall C., Fackler M.J. , Schwartz J.F., Shaper J.H. (1984) Antigenic analysis of haematopoesis. III. A hematopoetic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-Ia cells. J. Immunol. 133; 157-165. 2. Civin C.I., Trischmann T.M., Fackler M.J., Bernstein I.D., Bühring H.J., Campos L., et al. (1989) M7.1. Report on the CD34 cluster workshop. In: Kapp W. et al. Eds. Leucocyte Typing IV. White Cell Differentiation Antigens. Oxford, New York, Tokyo: Oxford University Press pp. 818-825. 3. Fina L. et al. (1990) Expression of the CD34 gene in vascular endothelial cells. Blood 75; 2417-2426. 4. Dercksen M.W., Daams G.M., de Haas M., von dem Borne A.E.G.K., and van der Schoot C.E. M10.2. Charakterization of the CD34 cluster. In: Schlossman S.F. et al. Eds. Leucocyte Typing V. White Cell Differentiation Antigens. Oxford, New York, Tokyo: Oxford University Press pp. 850-853.