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CD31 (PECAM-1), human


Catalogue number: CD31-5

CloneJC70A
IsotypeIgG1
Units5 ml
HostMouse
Application IHC(C
P)
Background
The antibody JC70A primarily is used for the immunohistological detection of endothelial cells in Formalin-fixed tissues. Positive reactions are expected as well in normal as in transformed tissue. The antibody frequently is used for evaluation of angiogenesis in proliferating tumours (e.g. in mamma carcinoma, colorectal carcinoma and lung cancer). Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1) or CD31 belongs to a group of transmembrane glycoproteins of the immunoglobulin super-family of adhesion molecules. It is exprimed predominantly by megakaryocytes, platelets, granulocytes, B-cell precursors and endothelial cells. It plays an important role in adhesion of leucocytes to blood vessel endothelia and for the cell migration through the vessel walls. Human CD31, Formalin-resistant epitope on a 130 kDa glycoprotein chain, reactivity confirmed on V. Internat. Workshop on Leucocyte Typing (Boston 1993).

Positive Control
Tonsil

Pre-Treatment
Pre-treatment of formalin-fixed, paraffin-embedded tissues at high temperature with citrate buffer (art. no. DE000) or better with TRIS-buffered EDTA (art. no. DE006).

Immunogen
Spleen cell membrane preparation of hairy cell leukaemia affected patient

Purification Method
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Working Concentration
(RTU) neat

Incubation Time
60 min at RT

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Applications
IHC(C, P)

Cross Reactivity
Human

Storage
2-8°C

References
1. Parums D.V., Cordell J.L., Micklem K., Heryet A.R., Gatter K.C., and Mason D.Y. (1990) JC70: a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections. J. Clin. Pathol. 43; 752-757. 2. Newman P.J., and Paddock C. (1995) CD31 cluster workshop report. In: Schlossman S.F. et al. Leucocyte Typing V. White Cell Differentiation Antigens. Oxford University Press, Oxford, N.Y., Tokyo pp 1259-1260. 3. Liao F., Huynh H.K., Eiora A., Greene T., Polizzi E., Muller W.A. (1995) Migration of monocytes across endothelium and passage through extracellular matrix involve separate molecular domains of PECAM 1. J. Exp. Med. 182; 1337-1343. 4. Buckley C.D., Doyannas R., Newton J.P., Blystone S.D., Brown E.J., Watt S.M., et al. (1996) Identification of alpha v and beta 3 as a heterotypic ligand for CD31/PECAM-1. J. Cell Sci. 109; 437-445.

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

CD31 (PECAM-1), human

$269.00

SKU: CD31-5 Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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