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CD30, human


Catalogue number: CD30-1

CloneHRS4
IsotypeIgG1
Units1 ml
HostMouse
Application IHC(P)
Background
The CD30/Ki-1 antigen is expressed by mononuclear Hodgkin cells and by multinucleated Reed-Sternberg cells in Hodgkin's disease. It is also present on tumour cells of a majority of anaplastic large cell lymphoma (ALL) and a varying proportion of activated T and B cells. App. one third of CD30+ cells lack LCA (CD45) expression. Human CD30 is a membrane glycoprotein, which is synthesized as a 90 kDa precursor protein which is processed by the Golgi apparatus into a phosphorylated membrane glycoprotein of 105/120 kDa. Human CD30/Ki-1 membrane glycoprotein 105/120 kDa..

Positive Control
Hodgkin's lymphoma tissue

Pre-Treatment
Pre-treatment of formalin-fixed, paraffin-embedded tissues at high temperature with citrate buffer (art. no. DE000) or with TRIS-buffered EDTA (art. no. DE006).

Immunogen
Recombinant CD30, extracellular domain

Purification Method
Protein G-purified antibody solution in PBS pH 7.3 with BSA and sodium azide (0.09%)

Working Concentration
(liquid conc.) 1:10-1:50

Concentration
50 µg/ml

Incubation Time
60 min at RT

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Product
Protein G-purified antibody solution in PBS pH 7.3 with BSA and sodium azide (0.09%)

Applications
IHC(P)

Cross Reactivity
Human

Storage
2-8°C

References
1. Schwarting R. et al. Blood, 1989, 74(5); 1678-1689

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

CD30, human

$553.00

SKU: CD30-1 Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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