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CD1a, human (MTB1)


Catalogue number: CD1A-MTB

CloneMTB1
IsotypeIgG1,k
Units1 ml
HostMouse
Application IHC(P)
Background
Clone MTB1 labels cortical thymocytes, Langerhans-Cells in the skin as well as interdigitating cells in the dermis and in squamous epithelia of tonsils. It labels also a small group of cells outside the germinal center of tonsils, which indicates a possible cross-reactivity with CD1b. Up to now CD1 has been identified as five different variants encoded by 5 different genes (they are designed as CD1a, b, c, d and e). CD1 is exprimed by cortical thymocytes, Langerhans cells in epithelia and dendritic cells. Usually it doesn't occur in mature peripheral blood cells, but is exprimed intra-cytoplasmatically in activated T-cells. CD1a plays a role in the detection of graft versus host reaction, in the detection of interdigitating cells in artereosclerotic lesion and in asthmatic inflammation. Human CD1a, possible cross-reactivity with CD1b.

Positive Control
Thymus

Pre-Treatment
Pre-treatment of formaldehyde-fixed material should be treated with Unmasking Solution E, TRIS-buffered EDTA pH 9 (Art. No. DE006) at 96-100°C

Immunogen
Recombinant protein corresponding to full length extracellular CD1a domain

Purification Method
Cell culture supernatant containing 0.09% sodium azide as preservative

Working Concentration
(liquid conc.) 1:20-1:40

Incubation Time
60 min at RT

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005).

Product
Cell culture supernatant containing 0.09% sodium azide as preservative

Applications
IHC(P)

Cross Reactivity
Human

Storage
2-8°C

References
1. Hunger RE, Sieling PA, Ochoa MT, Sugaya M, Burdick AE, Rea TH, Brennan PJ, Belisle JT, Blauvelt A, Porcelli SA, Modlin RL. Langerhans cells utilize CD1a and langerin to efficiently present nonpeptide antigens to T cells. J Clin Invest. 2004 Mar;113(5):701-8. 2. Takagi S, Miyagawa S, Ichikawa E, Soeda J, Miwa S, Miyagawa Y, Iijima S, Noike T, Kobayashi A, Kawasaki S. Dendritic cells, T-cell infiltration, and Grp94 expression in cholangiocellular carcinoma. Hum Pathol. 2004 Jul;35(7):881-6. 3. Poindexter NJ, Sahin A, Hunt KK, Grimm EA. Analysis of dendritic cells in tumor-free and tumor-containing sentinel lymph nodes from patients with breast cancer. Breast Cancer Res. 2004;6(4):R408-15. 4. Mazal PR, Hainfellner JA, Preiser J, Czech T, Simonitsch I, Radaszkiewicz T, Budka H. Langerhans cell histiocytosis of the hypothalamus: diagnostic value of immunohistochemistry. Clin Neuropathol. 1996 Mar-Apr;15(2):87-91. 5. Bobryshev YV, Lord RS. Langhans cells of human arterial intima: uniform by stellate appearance but different by nature. Tissue Cell. 1996 Apr;28(2):177-94.

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

CD1a, human (MTB1)

$901.00

SKU: CD1A-MTB Categories: ,

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Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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