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CD15, human


Catalogue number: CD15-1

CloneMMA/LeuM1
IsotypeIgM
Units1 ml
HostMouse
Application FACS
Flow Cytometry
IHC(P)
Background
The antibody against CD15 CD15 is a helpful tool for the identification of Reed-Sternberg-Cells in Hodgkin's disease. Occasionally CD15 is exprimed also in large cell B- and T-cell lymphoma. CD15 or X-Hapten plays a role in mediating phagocytosis, bacterial activity and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree monocytes. It is also present on Reed-Sternberg cells and on some epithelia. Human CD15 (3-fucosyl-N-acetyllactosamine).

Positive Control
Reed-Sternberg cells in Hodgkin's disease

Pre-Treatment
Formaldehyde-fixed material should be treated with Unmasking Solution C, citrate buffered solution pH 6 (Art. No. DE000) at 96-100°C followed by short term treatment with Pepsin (0.1%)

Immunogen
U937 histiocytic cell line

Purification Method
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Working Concentration
(liquid conc.) 1:15-1:25

Concentration
50 µg/ml

Incubation Time
60 min at RT

Secondary Reagents
As secondary reagents we recommend the use of biotinylated anti-mouse IgM antibody (Art. No. BA-2020) in combination with streptavidin conjugates or ABC systems.

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Applications
IHC(P), FACS

Cross Reactivity
Human

Storage
2-8°C

References
1. Skubitz K. et al. In: White Cell Differentiation Antigens. New York / Oxford University Press 1989; 800-805.

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

CD15, human

$395.00

SKU: CD15-1 Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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