Cathepsin DCatalogue number: CA111
Beside other factors like EGFR and hormone receptors, Cathepsin D is thought to be a prognostic factor in mamma carcinoma. It is indicative for tumour progression and invasion. Cathepsin D is a lysosomal enzyme that is involved in intracellular protein metabolism. It is synthesized as a 54 kDa precursor which is processed to a 46 kDa active enzyme product. It is involved in the metabolism of proteoglycans, cartilage collagen and in bone resorption. Human Cathepsin D.
Immunogen: Recombinant human Cathepsin D
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002.
Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002.
Concentration: 40 µg/ml
Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005).
Species Reactivity: Human
Incubation Time: 60 min at RT
Working Concentration: (liquid conc.) 1:10-1:50
Pre-Treatment: Pre-treatment with proteases is not recommended!
Positive Control: Breast carcinoma
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
1. Garcia M., et al. (1987) Immunohistochemical distribution of the 52-kDa protein in mammary tumours: a marker associated with cell proliferation rather than with hormone responsiveness. J. Steroid Biochem. 27(1): 439.
Database Name: UniProt
Accession number: P07339