Mouse anti Human Caspase-8Catalogue number: X1175M
|Clone||FLICE 1 1-37|
|Product Type||Monoclonal Antibody|
Caspases are key effectors of programmed cell death. They are synthesized as inactive proenzymes which are activated by cleavage at a specific aspartate residue to form two subunits. These subunits are normally linked together by a linker which may be involved in the regulation of the different caspases. Caspase-8 is a key enzyme in the apoptosis pathway. Caspase-8 contains two N-terminal region death effector domains which are removed to activate the enzyme along with cleavage into the two subunits. These subunits then for the active protease which is capable of cleaving caspase-3, -6 and -7. These caspases are then capable of cleaving other cellular substrates such as PARP and DFF, which in turn induce apoptosis.
Synonyms: Cysteine-requiring Aspartate Protease-8; Apoptotic protease Mch-5; ICE-like apoptotic protease 5; FADD-like ICE
Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human capase-8 protein and mouse myeloma cells.
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Detects proenzyme form of human Caspase-8 by Western blot analysis as well as one of the cleavage products. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: MCF-7 cell lysates
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
1. Cohen, G.M., et al. Caspases: the executioners of apoptosis. Biochem. J. 1997, 326, 1-16 2. Martin, D.A., et al. Membrane oligomerization and cleavage activates the caspase-8 (FLICE/MACHalpha1) death signal. J. Biol. Chem. 1998, 273, 4345-4349 3. Green, D.R., Apoptotic pathways: the roads to ruin. Cell 1998, 94, 695-698 4. Hu, S., et al. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J. Biol. Chem. 1997, 272, 17255-17257
Database Name: SwissProt
Accession number: Q14790
Species Accession: Human
Safety Datasheet(s) for this product:
Mouse anti Human Caspase-8