Mouse anti Human Caspase-7Catalogue number: X1267M
|Product Type||Monoclonal Antibody|
Caspases are key effectors of programmed cell death. Caspase-7 along with caspase 3 and 6 form the group of effector caspases that are responsible for the cleavage of multiple substrates including the cytokeratins, PARP, alpha fodrin, NuMA and others. Caspase-7 is a 303 amino acid protein with high similarity to caspase-3. Caspase-7 occurs in three varient forms. Granzyme B activates pro-caspase-7 to a form which can cleave poly(ADP-ribose) polymerase (PARP) to its signature fragment of ~85 kDa. Also, in vivo caspase-7 appears to be a better substrate for granzyme B than caspase-3. Pro-caspase-7 has been shown to exist as dimers or high order oligomers. Caspase-7 may be an important intracellular effector of granzyme B-mediated apoptosis and cytotoxic T-lymophocyte-induced cell killing in vivo.
Synonyms: Cysteine-requiring Aspartate Protease-7; Apoptotic protease Mch-3; ICE-like apoptotic protease 3
Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human caspase-7 protein and mouse myeloma cells.
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Detects proenzyme form of human Caspase-7 by Western blot. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: MCF-7 cell line
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
1. Cohen, G.M., et al. Caspases: the executioners of apoptosis. Biochem. J. 1997, 326, 1-16 2. Chandler, J.M., et al. Different subcellular distribution of Caspase-3 and Caspase-7 following Fas-induced apoptosis in mouse liver. J. Bio. Chem. 1998, 273, 10815-10818 3. Behrensdorf, H.A., et al. The endothelial monocyte-activating polypeptide II (EMAP II) is a substrate for caspase-7. FEBS Lett. 2000, 466, 143-147
Database Name: SwissProt
Accession number: P55210
Species Accession: Human
Safety Datasheet(s) for this product:
Mouse anti Human Caspase-7