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Calretinin, monoclonal


Catalogue number: CA130

Clone6B3
Isotypeig
Units1 ml
HostMouse
Application IHC(C
Immunoblotting
P)
Background
The antibody is suitable for the detection of Calretinin in neurons and may be used as an aid in differential diagnosis of pleura-mesothelioma. This marker is frequently expressed in epitheloid mesothelioma, while occasionally it turns positive in sarcomatous mesothelioma and carcinoma. It was found to be negative in sarcoma (2,3) and in 90% of adenocarcinoma. Calretinin is a Calcium-binding protein related to Calmodulin and Calbindin. It is found in groups of neurons of the brain cortex, in the retina and in the spinal cord (1). More recent studies show that it is a suitable marker for mesothelioma of the lung (2, 3, 4, 5). Calretinin, no cross-reaction with Calbindin and Calmodulin.

Positive Control
Cerebral cortex, pleural mesothelioma

Pre-Treatment
Improvement of staining with Unmasking Fluid G (Art. No. DE007) or Unmasking Fluid C (Art. No. DE000)

Immunogen
Recombinant human Calretinin 22kDa

Purification Method
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for 200-500 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

Working Concentration
(liquid conc.) 1:40-1:50

Incubation Time
60 min at RT

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005).

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for 200-500 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

Applications
IHC(C, P), Immunoblotting

Cross Reactivity
Human, Rat

Storage
2-8°C

References
1. Schwaller B., Buchwald P., Blümcke I., Celio M.R., and Hunziker W. (1994) Charakterization of a polyclonal antiserum against the purified human recombinant calcium binding protein calretinin. Cell Calcium 14; 639-648. 2. Gotzos V., Vogt P., and Celio M.R. (1995) Calretinin is a selective marker for malignant pleural mesotheliomas of the epithelial type. Pathol. Res. Pract. 192; 137-147. 3. Doglioni C., Tos A.P., Laurino L., Iuzzolino P., Chiarelli C., Celio M.R., and Viale G. (1996) Calretinin: a novel immunohistochemical marker for mesothelioma. Am. J. Surg. Pathol. 20; 1037-1046. 4. Zimmermann L., and Schwaller B. (2002) Monoclonal antibodies recognizing epitopes of calretinins: dependence on Ca2+-binding status and differences in antigen accessibility in colon cancer cells. Cell Calcium 31; 13-25.

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

Calretinin, monoclonal

$427.00

SKU: CA130 Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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