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Mouse anti Human Calpain I (large subunit, p80)


Catalogue number: X1013

Synonyms
Mouse Anti-Calpain I, u-Calpain (Large subunit, p80), Monoclonal Antibody

Clone15C10
IsotypeIgG1
Product Type Monoclonal Antibody
Units100 µg
HostMouse
Species reactivity Bovine
Human
Mouse
Rat
Application Enzyme Immunoassay
Immunoprecipitation
Western Blot
Product Form
Unconjugated

Background
The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and µ-calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kD subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, µ-calpain is active at micromolar (uM) concentrations of Ca ++. In addition to the ubiquitously expressed m- and µcalpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.

Immunogen
Hybridoma produced by the fusion of splenocytes from mice immunized with human calpain I purified from placenta with mouse myeloma.

Purification Method
Protein A/G Chromatography

Concentration
See vial for concentration

Formulation
Provided as a solution containing phosphate buffered saline, 0.08% sodium azide and 50% glycerol.

Functional Analysis
Western Blotting

Applications
Application: Use at 0.5-1 ug/ml for ELISA and western blot, 1-2ug/ml for immunoprecipitation.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Antibodies are stable for one year from purchase if stored frozen

References
1. Adamec, E., et al. 'Calpain I activation in rat hippocampal neurons in culture is NMDA receptor selective and not essential for excitotoxic cell death.' Brain Res. Mol. Brain Res. 1998, 54, 35-48 2. Guttmann, R.P. et al. 'Oxidation inhibits substrate proteolysis by calpain I but not autolysis.' J. Biol. Chem. 1997, 272, 2005-2012

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Glycerol
Sodium Azide
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/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Mouse anti Human Calpain I (large subunit, p80)

$254.00


Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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