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Mouse anti Human Calpain I, II (small subunit), conjugated with agarose


Catalogue number: X1011

Synonyms
Mouse anti calpain I, II small subunit, p30, agarose conjugate

Clone28F3
IsotypeIgG1
Product Type Monoclonal Antibody
Units1 ml
HostMouse
Species reactivity Human
Application Immunoprecipitation
Product Form
Agarose

Background
The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and µ-calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, µ-calpain is active at micromolar (µM) concentrations of Ca ++. In addition to the ubiquitously expressed m- and µcalpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.

Immunogen
Hybridoma produced by the fusion of splenocytes from mice immunized with human calpain with mouse myeloma.

Purification Method
Protein A/G Chromatography

Concentration
See vial for concentration

Formulation
Provided as a concentration of 1 mg/ml in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50% glycerol, 3mM sodium azide.

Functional Analysis
Western Blotting

Applications
Application: Use at 0.5-1 ug/ml for ELISA and western blot, 1-2ug/ml for immunoprecipitation.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Room Temperature

Product Stability
Antibodies are stable for one year from purchase if stored frozen

References
1. Adamec, E., et al. 'Calpain I activation in rat hippocampal neurons in culture is NMDA receptor selective and not essential for excitotoxic cell death.' Brain Res. Mol. Brain Res. 1998, 54, 35-48 2. Guttmann, R.P. et al. 'Oxidation inhibits substrate proteolysis by calpain I but not autolysis.' J. Biol. Chem. 1997, 272, 2005-2012

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Glycerol
Sodium Azide
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/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Mouse anti Human Calpain I, II (small subunit), conjugated with agarose

$254.00


Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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