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Calcitonin


Catalogue number: CA102

CloneSP17
IsotypeIg
Units1 ml
HostRabbit
Application IHC(C
P)
Background
The antibody, which is specific for human Calcitonin, may be used for the detection of C-cells in the thyroid glands. It is also suitable for the investigation of C-cell hyperplasia and for the detection of medullary thyroid carcinoma in which calcitonin is over-expressed. Calcitonin is a peptide hormone of 32 aa length, which is produced by C-cells of the thyroid gland. The hormone is responsible for the homeostasis of Calcium in blood and for Calcium uptake into bone cells. Human Calcitonin .

Positive Control
Thyroid gland, Medullary Thyroid Carcinoma

Pre-Treatment
Improvement of staining with unmasking fluid G (Art. No. DE007) or unmasking fluid C (Art. No. DE000)

Immunogen
Synthetic human Calcitonin 1-32 (KLH-conjugate)

Purification Method
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002.

Working Concentration
(liquid conc.) 1:25-1:50

Incubation Time
60 min at RT

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005).

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002.

Applications
IHC(C, P)

Cross Reactivity
Human, Monkey, Dog, Mouse, Horse, Rat, Sheep, Pig, others not tested

Storage
2-8°C

References
1. Talerman A., Lindeman J., Kievit-Tyson P.A., et al. (1979) Demonstration of calcitonin and carcinoembryonic antigen (CEA) in medulary carcinoma of the thyroid (MCT) by immunoperoxidase technique. Histopathol. 3; 503-510. 2. Holm R., Nesland J.M., Attramadal A. et al. (1989) Null cell adenomas of the pituitary gland. An immunohistochmical study. J. Pathol. 153; 213-217. 3. Grunditz T., et al. (1986) Calcitonin gene-related peptide in thyroid nerve fibers and C-cells: effect on thyroid hormone secretion and response to hypercalcemia. Endocrinology 119; 2313-2324. 4. Luts A. et al. (1994) Calcitonin, CGRP and helodermin in endocrine cells of the developing rat lung. Regulatory Peptides 51; 121-129.

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

Calcitonin

$712.00

SKU: CA102 Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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