Print Friendly, PDF & Email


Bromodeoxyuridine (BrdU)


Catalogue number: X2834

Product Type Reagent
Units25 mg
Product Form
Unconjugated

Background
Bromo-deoxyuridine (BrdU) is a thymidine analog that is used in cell proliferation studies. BrdU in culture is incorporated into the DNA during DNA synthesis. Cellular incorporation of BrdU can be detected by anti-BrdU specific antibodies following membrane permeabilization by flow cytometry or immunohistochemistry. The molecular weight of BrdU is 307.1.

Concentration
See vial for concentration

Formulation
Provided as 5 x 5 mg in sterile filtered solution in phosphate buffered saline

Functional Analysis
Immunohistochemistry

Applications
Prior to BrdU immunostaining to detect proliferating cells it is necessary to label the cells or tissue with BrdU. Labeling can either be done in vitro for cell cultures or performed in vivo for experimental animals. Both protocols are provided.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

References
- M. Caroprese, M.G. Ciliberti, P. De Palo, A. Santillo, A. Sevi, M. Albenzio, 2018, Glucocorticoid effects on sheep peripheral blood mononuclear cell proliferation and cytokine production under in vitro hyperthermia,Journal of Dairy Science, ISSN 0022-0302

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Bromodeoxyuridine (BrdU)

$92.00


Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

Newsletter







Member