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Sheep anti BrdU (bromodeoxyuridine)

Catalogue number: A205P

sheep anti BrdU, Bromodeoxyuridine

Product Type Polyclonal Antibody
Units250 µg
Application Immunohistochemistry
Western Blot
Product Form

Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells. This antibody is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining.

Bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin.

Purification Method
Ammonium Sulfate Precipitation

See vial for concentration

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Functional Analysis
Western Blotting

Applications: Western blotting: 25 -100 µg/ml; Immunoprecipitation: 25-100 µg/ml; Immunohistochemistry: 25-100 µg/ml The antibody has been tested using immunoprecipitation against 5-methyl cytosine (5-MeC) and bromodeoxyuridine (BrdU) or a control (no antigen). At a concentration of 25 ug/ml, this product demonstrates 8-fold higher reactivity with 5-MeC versus BrdU. For best results, use product at 25-100µg/ml. Note: Denature DNA sample first so that bases are accessible to anti BrdU antibody.

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Room Temperature

Product Stability
Products are stable for one year from purchase when stored properly

1. Nakamura, S., et al. Application of bromodeoxyuridine (BrdU) and anti-BrdU monoclonal antibody for the in vivo analysis of proliferative characteristics of human leukemia cells in bone marrows. Oncology 1991, 48, 285-289 2. Wilson, G., Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine. Methods Mol. Biol. 1998, 80, 255-266 3. Gray, J. (Ed), Special Issue: Monoclonal antibodies against bromodeoxyuridine Cytometry 1985, Vol. 6(6)

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide

Sheep anti BrdU (bromodeoxyuridine)



Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370