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Rabbit anti Human Beta-secretase 2 (NT) (Asp1)

Catalogue number: X1136P

Beta-site APP-cleaving enzyme 2, Aspartyl protease 1, Membrane-associated aspartic protease 1, Memapsin-1, Aspartic-like protease 56 kDa, Down region aspartic protease

Product Type Antigen Immunoaffinity Purified Polyclonal
Units100 µg
Species reactivity Human
Application Western Blot
Product Form

Accumulation of the amyloid- (A ) plaque in the cerebral cortex is a critical event in the pathogenesis of AlzheimerÕs disease. A peptide is generated by proteolytic cleavage of the amyloid protein precursor (APP) at - and -sites by proteases. The long-sought secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2)1-4. A BACE homolog was recently cloned and designated BACE2, Asp1, DRAP (for Down region aspartic protease), and memapsin 14-9. BACE2 also cleaves APP at -site and at a different site within A 8. BACE2 locates on chromosome 21q22.3, the so-called ÔDown critical regionÕ, suggesting that BACE2 and A may also contribute to the pathogenesis of Down syndrome6,7

Positive Control
Human heart tissue lysate

Synthetic peptide corresponding to amino acids 44 to 59 of human BACE2

Purification Method
Antigen Immunoaffiinity Purification

See vial for concentration

Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Functional Analysis
Western Blotting

Detects BACE2 by Western blot at 0.5 to 1 µg/ml. Detects an approximately 70 kDa band in human heart tissue lysate. Optimal concentration should be evaluated by serial dilutions.

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

1. Vassar, R., et al. Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999, 286, 735-741 2. Hussain, I., et al. Identification of a novel aspartic protease (Asp 2) as beta-secretase. Mol. Cell Neurosci. 1999, 14, 419-427 3. Yan, R., et al. Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity. Nature 1999, 402, 533-537 4. Sinha, S., et al. Purification and cloning of amyloid precursor protein beta-secretase from human brain. Nature 1999, 402, 537-540 5. Lin, X., et al. Human aspartic protease memapsin 2 cleaves the beta-secretase site of beta-amyloid precursor protein. Proc. Natl. Acad. Sci. USA 2000 15, 97, 1456-1460 6. Acquati, F., et al. The gene encoding DRAP (BACE2), a glycosylated transmembrane protein of the aspartic protease family, maps to the down critical region. FEBS Lett. 2000, 468, 59-64 7. Solans, A., et al. A new aspartyl protease on 21q22.3, BACE2, is highly similar to Alzheimer's amyloid precursor protein beta-secretase. Cytogenet. Cell Genet. 2000, 89, 177-184 8. Farzan, M., et al. BACE2, a beta -secretase homolog, cleaves at the beta site and within the amyloid-beta region of the amyloid-beta precursor protein. Proc. Natl. Acad. Sci. USA 2000, 97, 9712-9717 9. Bennett, B.D., et al. Expression analysis of BACE2 in brain and peripheral tissues. J. Biol. Chem. 2000, 275, 20647-20651

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide

Rabbit anti Human Beta-secretase 2 (NT) (Asp1)