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Rabbit anti Human Beta-secretase 2 (NT) (Asp1)


Catalogue number: X1136P

IsotypeIgG
Product Type Antigen Immunoaffinity Purified Polyclonal
Units100 µg
HostRabbit
Species reactivity Human
Mouse
Rat
Application Western Blot

Background
Accumulation of the amyloid- (A ) plaque in the cerebral cortex is a critical event in the pathogenesis of AlzheimerÕs disease. A peptide is generated by proteolytic cleavage of the amyloid protein precursor (APP) at - and -sites by proteases. The long-sought secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2)1-4. A BACE homolog was recently cloned and designated BACE2, Asp1, DRAP (for Down region aspartic protease), and memapsin 14-9. BACE2 also cleaves APP at -site and at a different site within A 8. BACE2 locates on chromosome 21q22.3, the so-called ÔDown critical regionÕ, suggesting that BACE2 and A may also contribute to the pathogenesis of Down syndrome6,7

Synonyms: Beta-site APP-cleaving enzyme 2, Aspartyl protease 1, Membrane-associated aspartic protease 1, Memapsin-1, Aspartic-like protease 56 kDa, Down region aspartic protease

Source

Immunogen: Synthetic peptide corresponding to amino acids 44 to 59 of human BACE2

Product

Product Form: Unconjugated

Formulation: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

Applications
Detects BACE2 by Western blot at 0.5 to 1 µg/ml. Detects an approximately 70 kDa band in human heart tissue lysate. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Human heart tissue lysate

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Ship Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

References
1. Vassar, R., et al. Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999, 286, 735-741 2. Hussain, I., et al. Identification of a novel aspartic protease (Asp 2) as beta-secretase. Mol. Cell Neurosci. 1999, 14, 419-427 3. Yan, R., et al. Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity. Nature 1999, 402, 533-537 4. Sinha, S., et al. Purification and cloning of amyloid precursor protein beta-secretase from human brain. Nature 1999, 402, 537-540 5. Lin, X., et al. Human aspartic protease memapsin 2 cleaves the beta-secretase site of beta-amyloid precursor protein. Proc. Natl. Acad. Sci. USA 2000 15, 97, 1456-1460 6. Acquati, F., et al. The gene encoding DRAP (BACE2), a glycosylated transmembrane protein of the aspartic protease family, maps to the down critical region. FEBS Lett. 2000, 468, 59-64 7. Solans, A., et al. A new aspartyl protease on 21q22.3, BACE2, is highly similar to Alzheimer's amyloid precursor protein beta-secretase. Cytogenet. Cell Genet. 2000, 89, 177-184 8. Farzan, M., et al. BACE2, a beta -secretase homolog, cleaves at the beta site and within the amyloid-beta region of the amyloid-beta precursor protein. Proc. Natl. Acad. Sci. USA 2000, 97, 9712-9717 9. Bennett, B.D., et al. Expression analysis of BACE2 in brain and peripheral tissues. J. Biol. Chem. 2000, 275, 20647-20651

Protein Reference(s)

Database Name: UniProt

Accession number: Q9Y5Z0 (Human)

Species Accession: Human


Safety Datasheet(s) for this product:
Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Rabbit anti Human Beta-secretase 2 (NT) (Asp1)

$407.00


Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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