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Rabbit anti Human Beta-secretase 1 (CT)


Catalogue number: X1135P

Synonyms
BACE, KIAA1149, Beta-site amyloid precursor protein cleaving enzyme 1

IsotypeIgG
Product Type Polyclonal Antibody
Units100 µg
HostRabbit
Species reactivity Human
Mouse
Application Western Blot
Product Form
Unconjugated

Background
Accumulation of the amyloid- (A ) plaque in the cerebral cortex is a critical event in the pathogenesis of AlzheimerÕs disease. A peptide is generated by proteolytic cleavage of the amyloid protein precursor (APP) at - and -sites by two proteases. APP is first cleaved by secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for secretase to generate the 4 kDa amyloid- peptide, which is deposited in the brains of all suffers of AlzheimerÕs disease. The long-sought secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2)1-4. BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.

Positive Control
Human brain tissue lysate

Immunogen
Synthetic peptide corresponding to amino acids 485 to 501 of human BACE. Sequence differs from that of rat and mouse by one amino acid.

Purification Method
Antigen Immunoaffiinity Purification

Concentration
See vial for concentration

Formulation
Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Functional Analysis
Western Blotting

Applications
Detects BACE by Western blot at 0.5 to 1 µg/ml. Detects an approximately 70 kDa band in human brain tissue lysate. Optimal concentration should be evaluated by serial dilutions.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

References
1. Vassar, R., et al. Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999, 286, 735-741 2. Hussain, I., et al. Identification of a novel aspartic protease (Asp 2) as beta-secretase. Mol. Cell Neurosci. 1999, 14, 419-427 3. Yan, R., et al. Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity. Nature 1999, 402, 533-537 4. Sinha, S., et al. Purification and cloning of amyloid precursor protein beta-secretase from human brain. Nature 1999, 402, 537-540

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Rabbit anti Human Beta-secretase 1 (CT)

$399.00

SKU: X1135P Categories: ,