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Beta-Defensin-4 (MBD-4)


Catalogue number: MBD041-A

Units100 µg
HostRabbit
Application ELISA
IHC not established
RIA
WB
Western Blotting
Background
Mammalian defensins are Cys-rich antimicrobial peptides which specifically react against Gram-negative bacteria. Originally defensins were isolated from hemolysates, but they can also be found on the cell surface of epithelia of the urogenital tract, lung, and trachea. In leukocytes defensins are stored in granules being released into phagolysosomes for the destruction of incorporated microorganisms. Amino acid sequences of alpha-defensins 1-4 (HNP1-4) and beta-Defensins 1-4 (HBD1-4 and MBD-4) are known.

Immunogen
Synthetic peptide: 11 aa N-terminal sequence of MBD-4 coupled to KLH

Purification Method
Antigen-agarose purified IgG in PBS, BSA, lyophilized without preservative. Reconstitute with 0.1 ml sterile water to get an antibody concentration of 1 mg/ml. Dilution buffers are depending on the type of application.

Working Concentration
(lyophilisate) 1-10 µg/ml Western Blot using ECL technique , 0.5-1 µg/ml ELISA,IHC 2-10 µg/ml (not tested)

Product
Antigen-agarose purified IgG in PBS, BSA, lyophilized without preservative. Reconstitute with 0.1 ml sterile water to get an antibody concentration of 1 mg/ml. Dilution buffers are depending on the type of application.

Applications
WB, ELISA, RIA, IHC not established

Cross Reactivity
Mouse

Storage
-20°C

References
1. Jia H.P., Wowk S.A., Schutte B.C., Lee S.K., Vivado A., Tack B.F., Bevins C.L., McCray P.B. Jr. A novel murine beta-defensin expressed in tongue, esophagus, and trachea. J. Biol. Chem. 275:33314-33320(2000) [PubMed] [Europe PMC] 2. Mural R.J., Adams M.D., Myers E.W., Smith H.O. et al. A comparison of whole-genome shotgun-derived mouse chromosome 16 and the human genome. Science. 2002 May 31;296(5573):1661-71. 3. Robert A Dorschner, Kenneth H Lin, Masamoto Murakami and Richard L Gallo (2003) Neonatal Skin in Mice and Humans Expresses Increased Levels of Antimicrobial Peptides: Innate Immunity During Development of the Adaptive Response Pediatric Research 53, 566–572

Caution
These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

Beta-Defensin-4 (MBD-4)

$838.00

SKU: MBD041-A Categories: ,