Beta-Defensin-4 (MBD-4)Catalogue number: MBD041-A
IHC not established
Mammalian defensins are Cys-rich antimicrobial peptides which specifically react against Gram-negative bacteria. Originally defensins were isolated from hemolysates, but they can also be found on the cell surface of epithelia of the urogenital tract, lung, and trachea. In leukocytes defensins are stored in granules being released into phagolysosomes for the destruction of incorporated microorganisms. Amino acid sequences of alpha-defensins 1-4 (HNP1-4) and beta-Defensins 1-4 (HBD1-4 and MBD-4) are known.
Immunogen: Synthetic peptide: 11 aa N-terminal sequence of MBD-4 coupled to KLH
Antigen-agarose purified IgG in PBS, BSA, lyophilized without preservative. Reconstitute with 0.1 ml sterile water to get an antibody concentration of 1 mg/ml. Dilution buffers are depending on the type of application.
Purification Method: Antigen-agarose purified IgG in PBS, BSA, lyophilized without preservative. Reconstitute with 0.1 ml sterile water to get an antibody concentration of 1 mg/ml. Dilution buffers are depending on the type of application.
Species Reactivity: Mouse
WB, ELISA, RIA, IHC not established
Working Concentration: (lyophilisate) 1-10 µg/ml Western Blot using ECL technique , 0.5-1 µg/ml ELISA,IHC 2-10 µg/ml (not tested)
These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
1. Jia H.P., Wowk S.A., Schutte B.C., Lee S.K., Vivado A., Tack B.F., Bevins C.L., McCray P.B. Jr. A novel murine beta-defensin expressed in tongue, esophagus, and trachea. J. Biol. Chem. 275:33314-33320(2000) [PubMed] [Europe PMC] 2. Mural R.J., Adams M.D., Myers E.W., Smith H.O. et al. A comparison of whole-genome shotgun-derived mouse chromosome 16 and the human genome. Science. 2002 May 31;296(5573):1661-71. 3. Robert A Dorschner, Kenneth H Lin, Masamoto Murakami and Richard L Gallo (2003) Neonatal Skin in Mice and Humans Expresses Increased Levels of Antimicrobial Peptides: Innate Immunity During Development of the Adaptive Response Pediatric Research 53, 566–572
Database Name: UniProt
Accession number: P82019 (DEFB4_MOUSE)
Species Accession: Mouse