Beta-Defensin-3 (HBD-3/MBD-3)Catalogue number: DEF03-S
|Product Type||Primary Antibodies|
The peptide sequences of HBD-3/MBD-3 used for immunisation contain very little consensus aa with any of the other human or mouse defensins. Human defensins are Cys-rich antimicrobial peptides which specifically react against Gram-negative bacteria. Originally defensins were isolated from hemolysates, but they can also be found on the cell surface of epithelia of the urogenital tract, lung, and trachea. In leukocytes defensins are stored in granules being released into phagolysosomes for the destruction of incorporated micro-organisms. Amino acid sequences of alpha-defensins 1-4 (HNP1-4) and beta-Defensins 1-4 (HBD1-4) are known. Mature Human Beta-Defensin-3 (HBD-3) shares app. 22% aa sequence homology with HBD-1. ß-defensin-3 of human and mouse origin, N-terminal fragments of HBD-3 and MBD-3, no cross-reactivity with other known ß-defensins or alpha-defensins.
Synonyms: Beta-Defensin-3 (HBD-3/MBD-3)
Rabbits were immunized with a mixture of synthetic peptides 15 aa near C-terminus of the mature HBD-3 (LPKEEQIGKCSTRGR) and 13 aa of N-terminal MBD-3, coupled to KLH.
Rabbit serum lyophilized with PBS and sodium azide 0.05%. Reconstitute with 1 ml bidistilled sterile water.
Species Reactivity: Human, mouse
ELISA, RIA, and WB. IHC not established
Working Concentration: (lyophilisate) 1:1000 - 1:5000 WB, 1:10000 - 1:50000 ELISA
Positive Control: Skin and airway epithelia
The antibody may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Shipping Conditions: Ship at ambient temperature.
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
1. Harder J., Bartels J., Christophers E., and J.-M. Schröder (1997) Antibacterial peptide specific for Gram-negative Bacteria / also effective for Candida albicans. Nature 387; 861ff
2. Bals R., Wang X., Meegalla R. L., Wattler S., et al. (1999) Mouse ß-Defensin 3 is an inducible antimicrobial peptide expressed in the epithelia of multiple organs. Infection and Immunity 67(7); 3542-3547.
3. Jia H.P., Schutte B.C., Schudy A., Linzmeier R., et al. (2001) Discovery of new human beta-defensins using a genomics-based approach. Gene 263(1-2);211-218.
4. Garcia J.R., Jaumann F., Schulz S., Krause A., et al. (2001) Identification of a novel multifunctional beta-defensin (human beta-defensin 3) with specific antimicrobial activity. Its interaction with plasma membranes of Xenopus oocytes and the induction of macrophage chemoattraction. Cell Tissue Res. 306(2); 257-264.
5. Harder J., Bartels J., Christophers E., and J.-M. Schröder (2001) Isolation and characterization of human beta-defensin-3, a novel human inducible peptide antibiotic. J. Biol. Chem. 276(8); 5707-5713.
Safety Datasheet(s) for this product:
/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf