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Beta-Defensin-2 (HBD-2/MBD-2)


Catalogue number: DEF02-S

Units100 µl
HostRabbit
Application ELISA
IHC not established
RIA

Background
Human defensins are Cys-rich antimicrobial peptides which specifically react against Gram-negative bacteria. Originally defensins were isolated from hemolysates, but they can also be found on the cell surface of epithelia of the urogenital tract, lung, and trachea. In leukocytes defensins are stored in granules being released into phagolysosomes for the destruction of incorporated microorganisms. Amino acid sequences of alpha-defensins 1-4 (HNP1-4) and beta-Defensins 1-4 (HBD1-4) are known. Beta-defensin-1 and beta-defensin-2 share 34% amino acid sequence homology. ß-defensin-2 (HBD-2) whole molecule and N-terminal fragments of HBD-2, no cross-reactivity to alpha-defensin and other beta-defensins, full cross-reaction with mouse and rat beta-defensin-2.

Source

Immunogen: Mixture of synthetic peptides (14 AA near N-terminus of the mature HBD-2, 14 AA near N-terminus of the mature rat ß-Defensin-2, and 17 AA near N-terminus of the mature mouse ß-Defensin-2) coupled to KLH for immunisation of rabbits

Product
Rabbit serum lyophilized with PBS and sodium azide 0.05%

Purification Method: Rabbit serum lyophilized with PBS and sodium azide 0.05%

Specificity

Species Reactivity: Human, mouse, rat, other species not tested

Applications
ELISA, RIA, IHC not established

Working Concentration: (lyophilisate) 1:1000 - 1:5000 WB, 1:10000 - 1:50000 ELISA

Positive Control: Skin or oral mucosa

Storage
-20'°C'

Caution
These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

References
1. Harder J., Bartels J., Christophers E., and J.-M. Schröder (1997) Antibacterial peptide specific for Gram-negative Bacteria / also effective for Candida albicans. Nature 387; 861ff 2. Hiratsuka T., Nakazato M., Date Y., Ashitani J., Minematsu T, Chino N., and S. Matsukura (1998) Biochem. Biophys. Res. Comm. (Pharmacol.) 249; 943 ff


Beta-Defensin-2 (HBD-2/MBD-2)

$807.00

SKU: DEF02-S Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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