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Beta-Defensin-1 (MBD-1)


Catalogue number: MBD011-S

Units100 µl
HostRabbit
Application ELISA
IHC not established
RIA
Background
The peptide fragment of MBD-1 used for immunisation shows 87% sequence homology rat beta-defensin-1 but no homology to other known defensins. Mammalian defensins are Cys-rich antimicrobial peptides which specifically react against Gram-negative bacteria. Originally defensins were isolated from hemolysates, but they can also be found on the cell surface of epithelia of the urogenital tract, lung, and trachea. In leukocytes defensins are stored in granules being released into phagolysosomes for the destruction of incorporated microorganisms. Amino acid sequences of alpha-defensins 1-4 (HNP1-4) and beta-Defensins 1-4 (HBD1-4) are known. Beta-defensin-1 and beta-defensin-2 share 34% amino acid sequence homology. Mouse beta-defensin-11 (MBD-1), cross-reactivity with rat expected, no cross-reaction with HBD-1.

Positive Control
Kidney or bladder epithelia

Immunogen
Synthetic 16 aa N-terminal peptide of mouse beta-defensin-1

Purification Method
Rabbit serum lyophilized with PBS and sodium azide 0.05%

Working Concentration
(lyophilisate) 1:1000 - 1:5000 WB, 1:10000 - 1:50000 ELISA

Product
Rabbit serum lyophilized with PBS and sodium azide 0.05%

Applications
ELISA, RIA, IHC not established

Cross Reactivity
Mouse

Storage
-20'°C'

References
1. Huttner KM, Kozak CA, Bevins CL. The mouse genome encodes a single homolog of the antimicrobial peptide human beta-defensin 1. FEBS Lett. 1997 Aug 11;413(1):45-49. 2. Ganz T. Defensins and host defense. Science. 1999 Oct 15;286(5439):420-1.

Caution
These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

Beta-Defensin-1 (MBD-1)

$791.00

SKU: MBD011-S Categories: ,