Beta-Defensin-1 (MBD-1), affinity purifiedCatalogue number: MBD011-A
IHC not established
The peptide fragment of MBD-1 used for immunisation shows 87% sequence homology rat beta-defensin-1 but no homology to other known defensins. Mammalian defensins are Cys-rich antimicrobial peptides which specifically react against Gram-negative bacteria. Originally defensins were isolated from hemolysates, but they can also be found on the cell surface of epithelia of the urogenital tract, lung, and trachea. In leukocytes defensins are stored in granules being released into phagolysosomes for the destruction of incorporated microorganisms. Amino acid sequences of alpha-defensins 1-4 (HNP1-4) and beta-Defensins 1-4 (HBD1-4) are known. Beta-defensin-1 and beta-defensin-2 share 34% amino acid sequence homology. Mouse beta-defensin-11 (MBD-1), cross-reactivity with rat expected, no cross-reaction with HBD-1.
Immunogen: Synthetic 16 aa N-terminal peptide of mouse beta-defensin-1
Peptide Sepharose purified IgG in PBS, BSA, lyophilized without preservative. Reconstitute with 0.1 ml sterile water to get an antibody concentration of 1 mg/ml. Dilution buffers are depending on the type of application.
Purification Method: Peptide Sepharose purified IgG in PBS, BSA, lyophilized without preservative. Reconstitute with 0.1 ml sterile water to get an antibody concentration of 1 mg/ml. Dilution buffers are depending on the type of application.
Species Reactivity: Mouse
ELISA, RIA, IHC not established
Working Concentration: (lyophilisate) 1-10 µg/ml Western Blot, 0.5-1 µg/ml ELISA, IHC 2-10 µg/ml (not tested)
Positive Control: Kidney or bladder epithelia
These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
1. Huttner KM, Kozak CA, Bevins CL. The mouse genome encodes a single homolog of the antimicrobial peptide human beta-defensin 1. FEBS Lett. 1997 Aug 11;413(1):45-49. 2. Ganz T. Defensins and host defense. Science. 1999 Oct 15;286(5439):420-1.
Beta-Defensin-1 (MBD-1), affinity purified