Beta-Defensin-1 (HBD-1)Catalogue number: DEF01-S
Human defensins are Cys-rich antimicrobial peptides which specifically react against Gram-negative bacteria. Originally defensins were isolated from hemolysates, but they can also be found on the cell surface of epithelia of the urogenital tract, lung, and trachea. In leukocytes defensins are stored in granules being released into phagolysosomes for the destruction of incorporated microorganisms. Amino acid sequences of alpha-defensins 1-4 (HNP1-4) and beta-Defensins 1-4 (HBD1-4) are known. Beta-defensin-1 and beta-defensin-2 share 34% amino acid sequence homology. ß-defensin-1 (HBD-1) whole molecule and 17AA N-terminal fraction of HBD-1, no cross-reactivity to alpha-defensin and other beta-defensins.
Immunogen: Synthetic peptide, 17 AA near N-terminus of the mature HBD-1, coupled to KLH for immunisation of rabbits
Rabbit serum lyophilized with PBS and sodium azide 0.05%
Purification Method: Rabbit serum lyophilized with PBS and sodium azide 0.05%
Species Reactivity: human, monkey, no cross-reactivity with mouse and rat
IHC(C, P), ELISA, RIA
Working Concentration: (lyophilisate) 1:1000 - 1:5000 WB, 1:10000 - 1:50000 ELISA
Positive Control: Renal epithelia, bladder epithelia
These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
1. Bensch K.W., Raida M., Mägert H.J., Schulz-Knappe P., and W.G. Forssmann. HBD-1: a novel beta -defensin from human plasma. FEBS Lett 1995, 368; 331-335. (original publication HBD-1) 2. Valore EV, Park CH, Quayle AJ, Wiles KR, McCray PB, Ganz T. Human beta-defensin-1: an antimicrobial peptide of urogenital tissues. J Clin Invest. 1998 Apr 15;101(8):1633-42. 3. O'Neil DA, Porter EM, Elewaut D, Anderson GM, Eckmann L, Ganz T, Kagnoff MF. Expression and regulation of the human beta-defensins hBD-1 and hBD-2 in intestinal epithelium. J Immunol. 1999 Dec 15;163(12):6718-24. 4. Ganz T. Defensins and host defense. Science. 1999 Oct 15;286(5439):420-1. 5. Shi J, Zhang G, Wu H, Ross C, Blecha F, Ganz T. Porcine epithelial beta-defensin 1 is expressed in the dorsal tongue at antimicrobial concentrations. Infect Immun. 1999 Jun;67(6):3121-7.