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Beta-Defensin-1 (HBD-1)


Catalogue number: DEF01-S

Units100 µl
HostRabbit
Application ELISA
IHC(C
P)
RIA
Background
Human defensins are Cys-rich antimicrobial peptides which specifically react against Gram-negative bacteria. Originally defensins were isolated from hemolysates, but they can also be found on the cell surface of epithelia of the urogenital tract, lung, and trachea. In leukocytes defensins are stored in granules being released into phagolysosomes for the destruction of incorporated microorganisms. Amino acid sequences of alpha-defensins 1-4 (HNP1-4) and beta-Defensins 1-4 (HBD1-4) are known. Beta-defensin-1 and beta-defensin-2 share 34% amino acid sequence homology. ß-defensin-1 (HBD-1) whole molecule and 17AA N-terminal fraction of HBD-1, no cross-reactivity to alpha-defensin and other beta-defensins.

Positive Control
Renal epithelia, bladder epithelia

Immunogen
Synthetic peptide, 17 AA near N-terminus of the mature HBD-1, coupled to KLH for immunisation of rabbits

Purification Method
Rabbit serum lyophilized with PBS and sodium azide 0.05%

Working Concentration
(lyophilisate) 1:1000 - 1:5000 WB, 1:10000 - 1:50000 ELISA

Product
Rabbit serum lyophilized with PBS and sodium azide 0.05%

Applications
IHC(C, P), ELISA, RIA

Cross Reactivity
human, monkey, no cross-reactivity with mouse and rat

Storage
-20'°C'

References
1. Bensch K.W., Raida M., Mägert H.J., Schulz-Knappe P., and W.G. Forssmann. HBD-1: a novel beta -defensin from human plasma. FEBS Lett 1995, 368; 331-335. (original publication HBD-1) 2. Valore EV, Park CH, Quayle AJ, Wiles KR, McCray PB, Ganz T. Human beta-defensin-1: an antimicrobial peptide of urogenital tissues. J Clin Invest. 1998 Apr 15;101(8):1633-42. 3. O'Neil DA, Porter EM, Elewaut D, Anderson GM, Eckmann L, Ganz T, Kagnoff MF. Expression and regulation of the human beta-defensins hBD-1 and hBD-2 in intestinal epithelium. J Immunol. 1999 Dec 15;163(12):6718-24. 4. Ganz T. Defensins and host defense. Science. 1999 Oct 15;286(5439):420-1. 5. Shi J, Zhang G, Wu H, Ross C, Blecha F, Ganz T. Porcine epithelial beta-defensin 1 is expressed in the dorsal tongue at antimicrobial concentrations. Infect Immun. 1999 Jun;67(6):3121-7.

Caution
These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

Beta-Defensin-1 (HBD-1)

$791.00

SKU: DEF01-S Categories: ,