Beta-CateninCatalogue number: CTN001-G
Beta-Catenin is a 88 kDa protein which is exprimed in many different tissues. Normally it is located in the cytoplasm near the cell membrane. It is associated with E-Cadherin and it may be essential for the function of this protein. Mutations in Beta-Catenin Gen CTNNBT1 lead to a loss of membrane binding and to an accumulation of the protein in the nuclear region. Nuclear accumulation is found especially in fibromatous lesions of the breast and abdomen. Therefore the antibody has been used for differentiation of these lesions from other spindle cell tumors, which may occur in the named body regions. Nuclear Accumulation of beta-Catenin was also found in colo-rectal carcinoma. Beta-Catenin (CTNB1); Catenin (Cadherin-associated Protein) beta 1.
Immunogen: Synthetic peptide corresponding to a N-terminal aa sequence of human ß-Catenin
Cell culture supernatant with PBS, glycerol, BSA, and NaN3 (0,09%)
Purification Method: Cell culture supernatant with PBS, glycerol, BSA, and NaN3 (0,09%)
Secondary Reagents: Anti-rabbit IgG:Biotin conjugate (code no. ZU101) in conjunction with Streptavidin-HRPO (code no. ZU054)
Species Reactivity: Human, Mouse, other species not tested
IHC(C, P), WB
Working Concentration: (RTU) RTU
Pre-Treatment: Formalin-fixed materials require heat induced antigen retrieval with citrate buffer (Art. No. DE000) or TEC-Buffer pH 8 (Art. No. DE005). We recommend an unmasking procedure for 30 min at 100°C.
Positive Control: Fibromatosis, Colon Carcinoma
** The ß-Catenin antibody is registrated and produced under US Patent No. 5675063 and 7429487. These Reagents are research reagents for in vitro applications only. They are not intended for clinical diagnostik use. The preservative sodium azide has been added in very small quantities. Nevertheless, it may be harmful to health and the reagent should be used only by trained personnel. For waste disposal, please refer to national regulations.
1. Montgomery E, Torbenson MS, Kaushal M, Fisher C, Abraham SC.(2002) Beta-catenin immunohistochemistry separates mesenteric fibromatosis from gastrointestinal stromal tumor and sclerosing mesenteritis. Am J Surg Pathol. 2002 Oct;26(10):1296-301. 2. Montgomery E, Folpe AL. (2005) The diagnostic value of beta-catenin immunohistochemistry. Adv Anat Pathol. 2005 Nov;12(6):350-6. 3. Images of Beta-Catenin in normal mamma tissue and mamma adenoma may be found with the following link: http://www.cpl.colostate.edu/pathology/ihc/catenin/bcat/bcat.htm
Database Name: UniProt
Accession number: P35222 (CTNB1_HUMAN)
Species Accession: Human