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Beta-Catenin


Catalogue number: CTN001-G

CloneE247
IsotypeIgG
Units5 ml
HostRabbit
Application IHC(C
P)
WB
Western Blotting
Background
Beta-Catenin is a 88 kDa protein which is exprimed in many different tissues. Normally it is located in the cytoplasm near the cell membrane. It is associated with E-Cadherin and it may be essential for the function of this protein. Mutations in Beta-Catenin Gen CTNNBT1 lead to a loss of membrane binding and to an accumulation of the protein in the nuclear region. Nuclear accumulation is found especially in fibromatous lesions of the breast and abdomen. Therefore the antibody has been used for differentiation of these lesions from other spindle cell tumors, which may occur in the named body regions. Nuclear Accumulation of beta-Catenin was also found in colo-rectal carcinoma. Beta-Catenin (CTNB1); Catenin (Cadherin-associated Protein) beta 1.

Positive Control
Fibromatosis, Colon Carcinoma

Pre-Treatment
Formalin-fixed materials require heat induced antigen retrieval with citrate buffer (Art. No. DE000) or TEC-Buffer pH 8 (Art. No. DE005). We recommend an unmasking procedure for 30 min at 100°C.

Immunogen
Synthetic peptide corresponding to a N-terminal aa sequence of human ß-Catenin

Purification Method
Cell culture supernatant with PBS, glycerol, BSA, and NaN3 (0,09%)

Working Concentration
(RTU) RTU

Secondary Reagents
Anti-rabbit IgG:Biotin conjugate (code no. ZU101) in conjunction with Streptavidin-HRPO (code no. ZU054)

Product
Cell culture supernatant with PBS, glycerol, BSA, and NaN3 (0,09%)

Applications
IHC(C, P), WB

Cross Reactivity
Human, Mouse, other species not tested

Storage
2-8°C

References
1. Montgomery E, Torbenson MS, Kaushal M, Fisher C, Abraham SC.(2002) Beta-catenin immunohistochemistry separates mesenteric fibromatosis from gastrointestinal stromal tumor and sclerosing mesenteritis. Am J Surg Pathol. 2002 Oct;26(10):1296-301. 2. Montgomery E, Folpe AL. (2005) The diagnostic value of beta-catenin immunohistochemistry. Adv Anat Pathol. 2005 Nov;12(6):350-6. 3. Images of Beta-Catenin in normal mamma tissue and mamma adenoma may be found with the following link: http://www.cpl.colostate.edu/pathology/ihc/catenin/bcat/bcat.htm

Caution
** The ß-Catenin antibody is registrated and produced under US Patent No. 5675063 and 7429487. These Reagents are research reagents for in vitro applications only. They are not intended for clinical diagnostik use. The preservative sodium azide has been added in very small quantities. Nevertheless, it may be harmful to health and the reagent should be used only by trained personnel. For waste disposal, please refer to national regulations.

Safety Datasheet(s) for this product:

Beta-Catenin

$395.00

SKU: CTN001-G Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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