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Sheep anti Human bcl-xl

Catalogue number: A110P

Product Type Polyclonal Antibody
Units100 µg
Species reactivity Human
Application Western Blot

bcl-x is a bcl-2-related gene that can function as a regulator of programmed cell death (apoptosis) independent of bcl-2. Alternative splicing results in two distinct bcl-x mRNAs. The larger mRNA gives rise to a protein product, bcl-xl, which is similar in size and predicted structure to bcl-2 (1). The smaller mRNA gives rise to bcl-xS. bcl-x immunoreactivity has been detected in a wide variety of cell types and the protein is typically present in the cytosol in association with the mitochondrial periphery, a property shared with bcl-2 however membrane bound forms of bcl-x have been demonstrated in thymocytes (2-4). Following the induction of apoptosis all of the bcl-x protein shifts to the membrane form (2). Of the two isoforms of bcl-x, the long (bcl-xl) is the most abundant mRNA species expressed in embryonic and adult tissues and most likely differs from bcl-2 in its regulatory activity on cell differentiation through controlled tissue specific expression (1,3). Like its homolog bcl-2, bcl-x undergoes phosphorylation, a modification that requires that a specific 60 amino acid loop region be intact, which in turn appears to regulate activity (5,6). Structurally, based on 3D-structure analysis, bcl-x forms pH sensitive cation-selective ion channels in membranes a property shared with the pore forming domains of several bacterial toxins (7). Bcl-xl has been shown to modify the cellÕs response to oxidants, to participate in resistance to chemotherapeutic agents and radiation, and to play a key role in the development of the developing CNS (8-10).

Synonyms: hbcl-xl, sheep anti human bcl-xl, bclxl, bcl xl, bclx, bcl2L1, BCL2L


Immunogen: A synthetic peptide corresponding to amino acids 3 to 14 of the human bcl-xl sequence


Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Application: Western Blot at 1-5 ug/ml. Positive control MCF-7 cells.

Functional Analysis: Western Blotting

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: room temperature

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

1. Boise L.H., et al. (1993) Cell 74: 597-608. 2. Krajewski S., et al. (1994) Cancer Res 54:5501-5507. 3. Gonzalez-Garcia M., et al. (1994) Development 120: 3033-3042. 4. Hsu, Y.T., et al. (1997) Proc. Natl. Acad. Sci USA 94: 3668-3672. 5. Benito, A., et al. (1997) Leukemia 11 940-944. 6. Chang, B.S., et al. (1997) EMBO J. 16: 968-977. 7. Minn, A.J., et al. (1997) Nature 385: 353-357. 8. Fang W., et al. (1995) J Immunol 155: 66-75. 9. Datta R., et al. (1995) Cell Growth Differ 6:363-370. 10. Shindler, K.S., et al. (1997) J. Neurosci. 17:3112-3119.

Protein Reference(s)

Database Name: UniProt

Accession number: Q07817 (Human)

Species Accession: Human

Safety Datasheet(s) for this product:
Sodium Azide

Sheep anti Human bcl-xl



Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370