Sheep anti Human bcl-xlCatalogue number: A110P
|Product Type||Polyclonal Antibody|
bcl-x is a bcl-2-related gene that can function as a regulator of programmed cell death (apoptosis) independent of bcl-2. Alternative splicing results in two distinct bcl-x mRNAs. The larger mRNA gives rise to a protein product, bcl-xl, which is similar in size and predicted structure to bcl-2 (1). The smaller mRNA gives rise to bcl-xS. bcl-x immunoreactivity has been detected in a wide variety of cell types and the protein is typically present in the cytosol in association with the mitochondrial periphery, a property shared with bcl-2 however membrane bound forms of bcl-x have been demonstrated in thymocytes (2-4). Following the induction of apoptosis all of the bcl-x protein shifts to the membrane form (2). Of the two isoforms of bcl-x, the long (bcl-xl) is the most abundant mRNA species expressed in embryonic and adult tissues and most likely differs from bcl-2 in its regulatory activity on cell differentiation through controlled tissue specific expression (1,3). Like its homolog bcl-2, bcl-x undergoes phosphorylation, a modification that requires that a specific 60 amino acid loop region be intact, which in turn appears to regulate activity (5,6). Structurally, based on 3D-structure analysis, bcl-x forms pH sensitive cation-selective ion channels in membranes a property shared with the pore forming domains of several bacterial toxins (7). Bcl-xl has been shown to modify the cellÕs response to oxidants, to participate in resistance to chemotherapeutic agents and radiation, and to play a key role in the development of the developing CNS (8-10).
Synonyms: hbcl-xl, sheep anti human bcl-xl, bclxl, bcl xl, bclx, bcl2L1, BCL2L
Immunogen: A synthetic peptide corresponding to amino acids 3 to 14 of the human bcl-xl sequence
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Application: Western Blot at 1-5 ug/ml. Positive control MCF-7 cells.
Functional Analysis: Western Blotting
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Shipping Conditions: room temperature
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
1. Boise L.H., et al. (1993) Cell 74: 597-608. 2. Krajewski S., et al. (1994) Cancer Res 54:5501-5507. 3. Gonzalez-Garcia M., et al. (1994) Development 120: 3033-3042. 4. Hsu, Y.T., et al. (1997) Proc. Natl. Acad. Sci USA 94: 3668-3672. 5. Benito, A., et al. (1997) Leukemia 11 940-944. 6. Chang, B.S., et al. (1997) EMBO J. 16: 968-977. 7. Minn, A.J., et al. (1997) Nature 385: 353-357. 8. Fang W., et al. (1995) J Immunol 155: 66-75. 9. Datta R., et al. (1995) Cell Growth Differ 6:363-370. 10. Shindler, K.S., et al. (1997) J. Neurosci. 17:3112-3119.
Database Name: UniProt
Accession number: Q07817 (Human)
Species Accession: Human
Safety Datasheet(s) for this product:
Sheep anti Human bcl-xl