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Sheep anti Mouse Bax-beta

Catalogue number: X1290P

Apoptosis regulator Bax cytoplasmic isoform beta , BaxB, BAX, BCL2 associated X protein, Apoptosis regulator BAX, BCL2 associated X protein isoform beta, Bax beta, Bax B

Product Type Polyclonal Antibody
Units250 µg
Species reactivity Mouse
Application Immunoprecipitation
Western Blotting
Product Form

Bax is encoded by a 1.5 kb RNA species created by the retention of intron 5 in the bax mRNA transcript. The exon4/intron5 splice junction is evolutionarily conserved and is found in both the human and mouse bax coding regions. The 630 bp intron 5 contributes 60 amino acids to the Bax protein before a stop codon is encountered during translation. The Bax protein is 24 kDa in size and although it contains the BH1, BH2, and BH3 domains identical to Bax, it has a unique carboxyl terminus and does not have a hydrophobic transmembrane domain (Oltvai et al., 1993). Bax mRNA has been found in many mouse tissues using RT-PCR. It is found co-expressed with Bax in lung, stomach, kidney, thymus, lymph node, bone marrow, pancreas, and spleen. Bax mRNA was very abundant in brain and duodenum with almost no Bax mRNA detected in these tissues (Oltvai et al., 1993).

Synthetic peptide corresponding to amino acids 172 to 199 of theBax protein

Purification Method
Ammonium Sulfate Precipitation

See vial for concentration

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Functional Analysis
Western Blotting

Antibody detects Baxβ protein by immunoprecipitation only. Will not detect denatured protein by Western blot. Optimal concentration should be evaluated by serial dilutions.

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

1. Oltvai, Z.N., et al. Bcl-2 Heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 1993, 74, 609-619.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide

Sheep anti Mouse Bax-beta



Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370