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Basal Cell Marker Cocktail 2, p63 Protein + HMW Cytokeratin


Catalogue number: BAS002-1.0

CloneEPR5701 + 34ßE12
IsotypeIgG + IgG1
Units1 ml
HostRabbit/Mouse
Application IHC(P)
Background
The p63 protein, a homologue of the tumour-suppressor p53, is highly expressed in the nuclei of basal or progenitor layer of many epithelial tissues. p63 is detected in prostate basal cells in normal prostate glands and PIN. Other markers for basal cells are high molecular cytokeratins (CK 1, 5, 10 and 14), which can be labelled with HMW CK clone 34ßE12. In prostate adenocarcinoma basal cells disappear and staining with both p63 and HMW CK fails. Thus the combination of p63 and HMW CK is a useful tool as differential markers for benign prostate glands and adenocarcinoma (negative marker). nuclear p63 and cytoplasmic high molecular weight cytokeratins.

Positive Control
Normal prostate tissue or skin

Pre-Treatment
Use formalin-fixed and paraffin-embedded sections; Retrieval conditions: Unmasking fluid T, TEC buffer (Tris/EDTA/Citrate) pH 8 (Art. No. DE005) in a pressure cooker at 100°C 20-40 minutes

Immunogen
Recombinant human p63 protein (aa 1-203) and cytokeratin extract of human stratum corneum

Purification Method
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**.

Working Concentration
(liquid conc.) 1:25-1:50

Incubation Time
30-60 min

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005), but also other staining systems including anti-rabbit IgG will be suitable.

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**.

Applications
IHC(P)

Cross Reactivity
Human

Storage
2-8°C

References
1. Signoretti S., Waltregny D., Dilks J., Isaak B., Lin D., Garraway L., et al. (2000) p63 is a prostate basal cell marker and is required for prostate development. Am J. Pathol. 157; 1769-1775. 2. Weinstein M.H., Signoretti S., and Loda M. (2002) Diagnostic utility of immunohistochemical staining for p63, a sensitive marker of prostatic basal cells. Mod. Pathol. 15(12); 1302-1308 3. Yang X.J., Lecksell K., Gaudin P., and Epstein J.I. (1999) Rare expression of high-molekular-weight cytokeratin in adenocarcinoma of the prostate gland: a study of 100 cases of metastatic and locally advanced prostate cancer. Am. J. Surg. Pathol. 23(2); 147-152. 4. Browne TJ, Hirsch MS, Brodsky G, Welch WR, Loda MF, Rubin MA. Prospective evaluation of AMACR (P504S) and basal cell markers in the assessment of routine prostate needle biopsy specimens. Hum Pathol. 2004 Dec;35(12):1462-8. 5. Jiang Z, Li C, Fischer A, Dresser K, Woda BA. Using an AMACR (P504S)/34betaE12/p63 cocktail for the detection of small focal prostate carcinoma in needle biopsy specimens. Am J Clin Pathol. 2005 Feb;123(2):231-6.

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

Basal Cell Marker Cocktail 2, p63 Protein + HMW Cytokeratin

$1,265.00

SKU: BAS002-1.0 Categories: ,