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Rabbit anti Human Autotaxin


Catalogue number: X1653P

IsotypeIgG
Product Type Polyclonal Antibody
Units100 µg
HostRabbit
Species reactivity Human
Application Enzyme Immunoassay
Western Blot

Background
Autotaxin (ATX), a tumor cell motility- stimulating protein is an ecto/exo-enzyme with significant homology to the plasma cell membrane differentiation antigen PC-1. ATX is a 99kDa core protein and may run as a 125-kDa glycoprotein when isolated from the human melanoma cell line (A2058). ATX, like PC-1 can hydrolyze the type I phosphodiesterase substrate p-nitrophenyl thymidine-5'-monophosphate. Autotaxin has a novel motility- regulating function for this class of ecto/exo-enzymes.

Synonyms: EctonucleotAutotaxin (ATX), a tumor cell motility- stimulating protein is an ecto/exo-enzyme with significant homology to the plasma cell membrane differentiation antigen PC-1. ATX is a 99kDa core protein and may run as a 125-kDa glycoprotein when isolate

Source

Immunogen: Synthetic peptide derived from the autotaxin protein

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (5-10 µg/ml) and EIA. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Human brain lysate (Cat. No. X1633C)

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Ship Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

References
[1] Murata J., Lee H.Y., Clair T., Krutzsch H.C., Arestad A.A., Sobel M.E., Liotta L.A., Stracke M.L.; cDNA cloning of the human tumor motility-stimulating protein, autotaxin, reveals a homology with phosphodiesterases.; J. Biol. Chem. 269:30479-30484(1994). [2] Lee H.Y., Murata J., Clair T., Polymeropoulos M.H., Torres R., Manrow R.E., Liotta L.A., Stracke M.L.; Cloning, chromosomal localization, and tissue expression of autotaxin from human teratocarcinoma cells.; Biochem. Biophys. Res. Commun. 218:714-719(1996). [3] Kawagoe H., Soma O., Goji J., Nishimura N., Narita M., Inazawa J., Nakamura H., Sano K.; Molecular cloning and chromosomal assignment of the human brain-type phosphodiesterase I/nucleotide.; Genomics 30:380-384(1995). [4] Strausberg R.L., et al Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.; Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).

Protein Reference(s)

Database Name: SwissProt

Accession number: Q13822

Species Accession: Human


Safety Datasheet(s) for this product:
Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Rabbit anti Human Autotaxin

$254.00


Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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