Print Friendly, PDF & Email


Rabbit anti Human AIM


Catalogue number: X1781P

Synonyms
Apoptosis inhibitor of macrophages, API6, API6, SP alpha, CD5L

IsotypeIgG
Product Type Polyclonal Antibody
Units100 µg
HostRabbit
Species reactivity Human
Mouse
Application Western Blot
Product Form
Unconjugated

Background
Apoptosis inhibitor of macrophages (AIM) is a member of the scavenger receptor cysteine-rich domain superfamily (SRCR-SF) initially identified as an inducible cell surface ligand of CD5. It was shown that AIM functions in the thymus as the inducer of resistance to apoptosis within CD4+/CD8+ thymocytes and as the supporter of the viability of these cells before thymic selection. AIM was also shown to support macrophage survival and enhance their phagocytic function. More recent experiments using recombinant AIM significantly inhibited apoptosis of NKT and T cells obtained from C. parvum-stimulated livers in vitro, suggesting that AIM functions to induce resistance to apoptosis in these cells and supports host defense against inflammation during infection.

Positive Control
Expressed in spleen, lymph node, thymus, bone marrow, and fetal liver, but not in non-lymphoid tissues.

Immunogen
Rabbit polyclonal AIM antibody was raised against a 13 amino acid peptide from near the amino terminus of human AIM.

Purification Method
Antigen Immunoaffiinity Purification

Concentration
See vial for concentration

Formulation
Provided as solution in phosphate buffered saline with 0.02% sodium azide

Functional Analysis
Western Blotting

Applications
AIM antibody can be used for the detection of AIM by Western blot at 0.5 – 1ug/ml. Optimal concentration should be evaluated by serial dilutions.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

References
1) Miyazaki T, Hirokami Y, Matsuhashi N, et al. Increased susceptibility of thymocytes to apoptosis in mice lacking AIM, a novel murine macrophage-derived soluble factor belonging to the scavenger receptor cysteine-rich domain superfamily. J. Exp. Med. 1999; 189:413-22. 2) Haruta I, Kato Y, Hashimoto E, et al. Association of AIM, a novel apoptosis inhibitory factor, with hepatitis via supporting macrophage survival and enhancing phagocytic function of macrophages. J. Biol. Chem. 2001; 276:22910 3) Kuwata K, Watanabe H, Jiang S-Y, et al. AIM inhibits apoptosis of T cells and NKT cells in Corynebacterium-induced granuloma formation in mice. Am. J. Path. 2003; 162:837-47.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Rabbit anti Human AIM

$399.00

SKU: X1781P Categories: ,