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Rabbit anti Human Apaf 1 (NT) Apoptosis Activating Protease Fact


Catalogue number: X1118P

IsotypeIgG
Product Type Polyclonal Antibody
Units100 µg
HostRabbit
Species reactivity Human
Mouse
Rat
Application Western Blot
Product Form
Unconjugated

Background
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 is ubiquitously expressed in human tissues (1).

Positive Control
Whole HeLa cell lysate

Immunogen
Synthetic peptide corresponding to amino acids 12 to 28 of the human Apaf-1 protein. The immunogen sequence is identical in human and mouse

Purification Method
Antigen Immunoaffiinity Purification

Concentration
See vial for concentration

Formulation
Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Functional Analysis
Western Blotting

Applications
Detects Apaf-1 by Western blot at 0.25 to 0.5 µg/ml. Detects a 130 kDa band in whole HeLa cell lysate. Optimal concentration should be evaluated by serial dilutions.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

References
1. Zou, H., Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997, 90, 405-13 2. Cecconi, F., et al., Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998, 94, 727-37 3. Li, P., et al., Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997, 91, 479-89 4. Hu, Y., et al., Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation. Proc Natl Acad Sci USA 1998, 95, 4386-91

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide
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Rabbit anti Human Apaf 1 (NT) Apoptosis Activating Protease Fact

$399.00


Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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