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Rabbit anti Human Amyloid Precursor Protein (APP) (NT)


Catalogue number: X1133P

Synonyms
Rabbit anti-β-Amyloid Peptide Precursor (Amyloid-β Peptide N-terminal)

IsotypeIgG
Product Type Polyclonal Antibody
Units100 µg
HostRabbit
Species reactivity Human
Mouse
Rat
Application Western Blot
Product Form
Unconjugated

Background
Accumulation of the amyloid- peptide (A ) in the cerebral cortex is a critical event in the pathogenesis of AlzheimerÕs disease. The amyloid protein precursor (APP) is cleaved by secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for secretase to generate the 4 kDa amyloid- peptide (A ), which is deposited in the brains of all suffers of AlzheimerÕs disease.

Positive Control
Murine brain lysate

Immunogen
Synthetic peptide corresponding to amino acids 653 to 662 of human amyloid A4 protein precursor (APP) or 1 to 10 of the 4kDa Amyloid- peptide generated by and -secretases. This sequence is identical to those of rabbit, pig, bovine, guinea pig and chicken.

Purification Method
Antigen Immunoaffiinity Purification

Concentration
See vial for concentration

Formulation
Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Functional Analysis
Western Blotting

Applications
Detects APP and Ab peptide by Western blot at 0.5 to 1 µg/ml. Detects an approximately 120 kDa band in human, mouse and rat brain tissue lysates. Optimal concentration should be evaluated by serial dilutions.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

References
1. Ponte, P., et al. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature 1988, 331, 525-527. 2. Selkoe, D.J. Cell biology of the amyloid beta-protein precursor and the mechanism of Alzheimer's disease. Annu. Rev. Cell Biol. 1994, 10, 373-403

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide
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Rabbit anti Human Amyloid Precursor Protein (APP) (NT)

$399.00