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Alpha-DEFENSIN (1-3), HNP1-3

Catalogue number: HNP103

CloneDEF-3
IsotypeIgG1
Units100 µg
HostMouse
Application Flow Cytometry
IHC(C
Immunohistochemistry (frozen)
Immunohistochemistry (paraffin)
P)

Background
The monoclonal antibody DEF-3 reacts with human alpha-defensins (HNP1-3), which are synthesized and stored by neutrophilic granulocytes. Human defensins are Cys-rich antimicrobial peptides which specifically react against Gram-negative bacteria. Originally defensins were isolated from hemolysates, but they can also be found on the cell surface of epithelia of the urogenital tract, lung, and trachea. In leukocytes defensins are stored in granules being released into phagolysosomes for the destruction of incorporated microorganisms. Amino acid sequences of alpha-defensins 1-4 (HNP1-4) and beta-Defensins 1-4 (HBD1-4) are known. Human Neutrophil Peptides (HNP1-3) syn. alpha-Defensins 1-3, reactivity with HNP-4 not tested.

Source

Immunogen: Native Defensins

Product
Affinity purified IgG, lyophilized from PBS, BSA, and sodium azide**, reconstitute with 0.5 ml sterile water to get a stock solution of 200 µg/ml.

Purification Method: Affinity purified IgG, lyophilized from PBS, BSA, and sodium azide**, reconstitute with 0.5 ml sterile water to get a stock solution of 200 µg/ml.

Concentration: 200 µg/ml IgG after reconstitution with 0,5 ml distilled water

Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Specificity

Species Reactivity: Human, cross-reactivity with rhesus monkey and primates expected

Applications
IHC(C, P), FACS

Working Concentration: (lyophilisate) 0.2 µg/ml IHC(C), 0.5 µg/ml IHC(P)

Pre-Treatment: Formaldehyde-fixed tissue requires antigen unmasking preferred methods are 0.1% Pronase, Proteinase K or Trypsin.

Positive Control: Infected skin or oral mucosa

Storage
-20'°C'

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References
1. Ganz T. et al. (1985) J. Clin. Invest., 76; 1427 ff 2. Ganz T., et al. (1992) Med. Microbiol. Immunol. 181; 99ff 3. Mizukawa N ; Sugiyama K ; Fukunaga J ; Ueno T ; Mishima K ; Takagi S ; Sugahara T (1998) Defensin-1, a peptide detected in the saliva of oral squamous cell carcinoma patients. Anticancer Res 18(6B):4645-4649. 4. Linzmeier R., Ho C.H., Hoang B.V. , and Ganz T. (1999) A 450 kb contig of defensin genes on human chromosome 8p23. Gene 233; 205-211. 5. Mikuzawa N. Sugiyama K., Kamio M., Yamachika E., Ueno T., Fukunaga J., Takagi S., Sugahara T. (2000) Immunohistochemical staining of human alpha-defensin-1 (HNP-1) in submandibular glands of patients with oral carcinomas. Anticancer Res. 20(2B); 1125-1127.


Safety Datasheet(s) for this product:
EA_Sodium Azide
/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf

Alpha-DEFENSIN (1-3), HNP1-3

$903.00

SKU: HNP103 Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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