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Alpha-DEFENSIN (1-3), HNP1-3


Catalogue number: HNP103

CloneDEF-3
IsotypeIgG1
Units100 µg
HostMouse
Application FACS
Flow Cytometry
IHC(C
P)
Background
The monoclonal antibody DEF-3 reacts with human alpha-defensins (HNP1-3), which are synthesized and stored by neutrophilic granulocytes. Human defensins are Cys-rich antimicrobial peptides which specifically react against Gram-negative bacteria. Originally defensins were isolated from hemolysates, but they can also be found on the cell surface of epithelia of the urogenital tract, lung, and trachea. In leukocytes defensins are stored in granules being released into phagolysosomes for the destruction of incorporated microorganisms. Amino acid sequences of alpha-defensins 1-4 (HNP1-4) and beta-Defensins 1-4 (HBD1-4) are known. Human Neutrophil Peptides (HNP1-3) syn. alpha-Defensins 1-3, reactivity with HNP-4 not tested.

Positive Control
Infected skin or oral mucosa

Pre-Treatment
Formaldehyde-fixed tissue requires antigen unmasking preferred methods are 0.1% Pronase, Proteinase K or Trypsin.

Immunogen
Native Defensins

Purification Method
Affinity purified IgG, lyophilized from PBS, BSA, and sodium azide**, reconstitute with 0.5 ml sterile water to get a stock solution of 200 µg/ml.

Working Concentration
(lyophilisate) 0.2 µg/ml IHC(C), 0.5 µg/ml IHC(P)

Concentration
200 µg/ml IgG after reconstitution with 0,5 ml distilled water

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Product
Affinity purified IgG, lyophilized from PBS, BSA, and sodium azide**, reconstitute with 0.5 ml sterile water to get a stock solution of 200 µg/ml.

Applications
IHC(C, P), FACS

Cross Reactivity
Human, cross-reactivity with rhesus monkey and primates expected

Storage
-20'°C'

References
1. Ganz T. et al. (1985) J. Clin. Invest., 76; 1427 ff 2. Ganz T., et al. (1992) Med. Microbiol. Immunol. 181; 99ff 3. Mizukawa N ; Sugiyama K ; Fukunaga J ; Ueno T ; Mishima K ; Takagi S ; Sugahara T (1998) Defensin-1, a peptide detected in the saliva of oral squamous cell carcinoma patients. Anticancer Res 18(6B):4645-4649. 4. Linzmeier R., Ho C.H., Hoang B.V. , and Ganz T. (1999) A 450 kb contig of defensin genes on human chromosome 8p23. Gene 233; 205-211. 5. Mikuzawa N. Sugiyama K., Kamio M., Yamachika E., Ueno T., Fukunaga J., Takagi S., Sugahara T. (2000) Immunohistochemical staining of human alpha-defensin-1 (HNP-1) in submandibular glands of patients with oral carcinomas. Anticancer Res. 20(2B); 1125-1127.

Caution
These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Alpha-DEFENSIN (1-3), HNP1-3

$885.00

SKU: HNP103 Categories: ,