Alpha-Actin, smooth muscleCatalogue number: AA500
The antibody 1A4 detects alpha-actin of smooth muscle origin, thus it is suitable to distinguish smooth muscle cells from cells of sarcomeric origin. It may also be used for differentiation from fibroblast actin (F-actin). Movement of cell organelles, cells, and organisms depends on the interaction of the cytoskeletal proteins actin and myosin. In birds and mammals up to now six different isoforms of actin have been identified using amino acid sequence analysis and electrophoresis. They are more or less specific for different organs resp. cell types. Four isoforms are known to occur in muscle cells, while two other forms are found in most other cell types. Alpha-Actin, smooth muscle.
Immunogen: Synthetic N-terminal decapeptide of smooth muscle Alpha-Actin
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Species Reactivity: Human and other vertebrates
Incubation Time: 60 min at RT
Working Concentration: (RTU) neat
Pre-Treatment: No protease pre-treatment required, but microwave treatment usually will intensify staining. We refer to BIOLOGO's Unmasking Fluid G (Art. No. DE007).
Positive Control: Intestine, Appendix
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
1. Skalli O, Ropraz P., Trzeciak A., Benzonana G., Gillessen D. Gabbiani G. (1986) A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J. Cell Biol. 103; 2787-2796 2. Skalli O., Schürch W., Seemayer T., Lagacé R., Montandon D., Pittet B., et al. (1989) Myofibroblasts from diverse pathologic settings are heterogenous in their content of actin isoforms and intermediate filament proteins. Lab Invest. 60; 275-285 3. Rønnov-Jessen L., Van Deurs B., Celis J.E., and Petersen O.W. (1990) Smooth muscle differentiation in cultured human breast gland stromal cells. Lab. Invest. 63; 532-543 4. Hasegawa T., Hirose T., Kudo E., Abe J., and Hizawa K. (1990) Cytosteletal characteristics of myofibroblasts in benign and reactive fibroblastic lesions. Virch. Arch. (A) Pathol. Anat. 416; 375-382
Database Name: UniProt
Accession number: P62736 (ACTA_HUMAN)
Species Accession: Human
Alpha-Actin, smooth muscle