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Alpha-Actin, smooth muscle


Catalogue number: AA500

Clone1A4
IsotypeIgG2a
Units5 ml
HostMouse
Application IHC(C
P)
Background
The antibody 1A4 detects alpha-actin of smooth muscle origin, thus it is suitable to distinguish smooth muscle cells from cells of sarcomeric origin. It may also be used for differentiation from fibroblast actin (F-actin). Movement of cell organelles, cells, and organisms depends on the interaction of the cytoskeletal proteins actin and myosin. In birds and mammals up to now six different isoforms of actin have been identified using amino acid sequence analysis and electrophoresis. They are more or less specific for different organs resp. cell types. Four isoforms are known to occur in muscle cells, while two other forms are found in most other cell types. Alpha-Actin, smooth muscle.

Positive Control
Intestine, Appendix

Pre-Treatment
No protease pre-treatment required, but microwave treatment usually will intensify staining. We refer to BIOLOGO's Unmasking Fluid G (Art. No. DE007).

Immunogen
Synthetic N-terminal decapeptide of smooth muscle Alpha-Actin

Purification Method
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

Working Concentration
(RTU) neat

Incubation Time
60 min at RT

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

Applications
IHC(C, P)

Cross Reactivity
Human and other vertebrates

Storage
2-8°C

References
1. Skalli O, Ropraz P., Trzeciak A., Benzonana G., Gillessen D. Gabbiani G. (1986) A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J. Cell Biol. 103; 2787-2796 2. Skalli O., Schürch W., Seemayer T., Lagacé R., Montandon D., Pittet B., et al. (1989) Myofibroblasts from diverse pathologic settings are heterogenous in their content of actin isoforms and intermediate filament proteins. Lab Invest. 60; 275-285 3. Rønnov-Jessen L., Van Deurs B., Celis J.E., and Petersen O.W. (1990) Smooth muscle differentiation in cultured human breast gland stromal cells. Lab. Invest. 63; 532-543 4. Hasegawa T., Hirose T., Kudo E., Abe J., and Hizawa K. (1990) Cytosteletal characteristics of myofibroblasts in benign and reactive fibroblastic lesions. Virch. Arch. (A) Pathol. Anat. 416; 375-382

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Alpha-Actin, smooth muscle

$237.00

SKU: AA500 Categories: ,