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Sheep anti Chicken Alk2


Catalogue number: X1482P

Synonyms
Activin Receptor-Like Kinase 2

IsotypeIgG
Product Type Polyclonal Antibody
Units250 µg
HostSheep
Species reactivity Chicken
Application Western Blot
Product Form
Unconjugated

Background
Activin Receptor-Like Kinase 2 is a member of the superfamily of type I receptors for transforming growth factor- (TGF- ). This receptor binds both activin and bone morphogenic proteins (BMPs). In mice, these receptors are expressed mainly in the extraembryonic visceral endoderm before gastrulation and later in both embryonic and extraembryonic cells during gastrulation. Also, it has been shown that ALK2 associates with and phosphorylates Smad1 and also regulated Smad5 and Smad8 phosphorylation. Additionally, it induces the Tlx2 promoter in the absense of BMP7.

Immunogen
Recombinant protein derived from the extracellular domain of the chicken ALK2 protein. Protein shares significant homology with the mouse, rat and human sequences.

Purification Method
Ammonium Sulfate Precipitation

Concentration
See vial for concentration

Formulation
Provided as solution in phosphate buffered saline with 0.08% sodium azide

Functional Analysis
Western Blotting

Applications
Antibody can be used for Western blotting (5-10 µg/ml), immunoprecipitation (5 µg/mg of protein lysate and immunohistochemistry on paraffin embedded tissue sections (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

References
1. Lai, Y.T., et al. 'Activin receptor-like kinase 2 can mediate atrioventricular cushion transformation.' Dev. Biol. 2000, 222, 1-11. 2. Gu, Z., et al. 'The type I serine/threonine kinase receptor ActRIA (ALK2) is required for gastrulation of the mouse embryo.' Development 1999, 126, 2551-2561. 3. Macias-Silva, M., et al. 'Specific activation of Smad1 signaling pathways by the BMP7 type I receptor, ALK2.' J. Biol. Chem. 1998, 273, 25628-25636

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Sheep anti Chicken Alk2

$249.00