Mouse anti Human AktCatalogue number: X1889M
|Product Type||Monoclonal Antibody|
Rat others not tested
AKT is a serine/threonine kinase that plays an important role in multiple biological responses including metabolism, cell survival and growth by phosphorylating many proteins including GSK-3 , caspase-9, BAD, and the Forkhead Transcription Factor. AKT is activated by PI3K, which in turn can interact with FAK and other cytoskeletal-associated proteins, thereby linking activation to the cytoskeleton. AKT/PKB is constitutively active in many types of human cancer, and contributes to tumor progression by promoting angiogenesis and cell invasiveness. AKT is fully activated by phosphorylation of threonine 308 by PDK1 and serine 473 by PDK2. The reactivity of this antibody with AKT2 and AKT3 has not been tested.
Synonyms: Protein Kinase B, RAC-alpha, EC 220.127.116.11, RAC-PK-alpha, PKB, C-AKT
Immunogen: Recombinant human AKT1 protein.
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline pH 7.2 with 0.1% BSA and 0.01% sodium azide
Purification Method: Purified from ascites by affinity chromatography.
Concentration: See vial for concentration
For Western blotting, the recommended concentration is 1 µg/mL Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Positive Control: Human Jurkat cells, mouse 3T3-L1 cells, and rat PC-12 cells. Found in all human cell types so far analyzed. Found in the cytoplasm (and in the nucleus after activation by integrin-linked protein kinase 1)
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Ship Conditions: Ship at ambient temperature, freeze upon arrival
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
1. Beffert, U., et al. (2002) Reelin-mediated signaling locally regulates protein kinase B/Akt and glycogen synthase kinase 3beta. J. Biol. Chem. 277(51):49958-49964. 2. Hsu, J., et al. (2001) The AKT kinase is activated in multiple myeloma tumor cells. Blood 98(9):2853-2855. 3. Persad, S., et al. (2001) Regulation of protein kinase B/Akt-threonine-308 phosphorylation by integrin linked kinase (ILK): Critical roles for kinase activity and amino acids arginine-211 and threonine-343. J. Biol. Chem. 276(29):27462-27469. 4. Nelson, J.M. and D.W. Fry (2001) Akt, MAPK (Erk1/2), and p38 act in concert to promote apoptosis in response to ErbB receptor family inhibition. J. Biol. Chem. 276(18):14842-14847. 5. Genot, E.M., et al. (2000) The T-cell receptor regulates Akt (Protein Kinase B) via a pathway involving Rac1 and phosphatidylinositide 3-kinase. Mol. Cell. Biol. 20(15):5469-5478. 6. Datta, S.R., et al. (1997) Akt phosphorylation of BAD couples survival signals to the cell- intrinsic death machinery. Cell 91(2):231-241. 7. del Peso, L., et al. (1997) Interleukin-3-induced phosphorylation of BAD through the protein kinase Akt. Science 278(5338):687-689. 8. Dudek, H., et al. (1997) Regulation of neuronal survival by the serine-threonine protein kinase Akt. Science 275(5300):661-665. 9. Franke, T.F., et al. (1997) PI3K: downstream AKT phosphorylation blocks apoptosis. Cell 88(4):435-437.
Database Name: UniProt
Accession number: P31749
Safety Datasheet(s) for this product:
Mouse anti Human Akt