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Mouse anti Human Akt

Catalogue number: X1889M

Clone9Q7
IsotypeIgG3
Product Type Monoclonal Antibody
Units100 µg
HostMouse
Species reactivity Human
Mouse
Rat others not tested
Application Western Blot

Background
AKT is a serine/threonine kinase that plays an important role in multiple biological responses including metabolism, cell survival and growth by phosphorylating many proteins including GSK-3 , caspase-9, BAD, and the Forkhead Transcription Factor. AKT is activated by PI3K, which in turn can interact with FAK and other cytoskeletal-associated proteins, thereby linking activation to the cytoskeleton. AKT/PKB is constitutively active in many types of human cancer, and contributes to tumor progression by promoting angiogenesis and cell invasiveness. AKT is fully activated by phosphorylation of threonine 308 by PDK1 and serine 473 by PDK2. The reactivity of this antibody with AKT2 and AKT3 has not been tested.

Synonyms: Protein Kinase B, RAC-alpha, EC 2.7.11.1, RAC-PK-alpha, PKB, C-AKT

Source

Immunogen: Recombinant human AKT1 protein.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline pH 7.2 with 0.1% BSA and 0.01% sodium azide

Purification Method: Purified from ascites by affinity chromatography.

Concentration: See vial for concentration

Applications
For Western blotting, the recommended concentration is 1 µg/mL Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Positive Control: Human Jurkat cells, mouse 3T3-L1 cells, and rat PC-12 cells. Found in all human cell types so far analyzed. Found in the cytoplasm (and in the nucleus after activation by integrin-linked protein kinase 1)

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

References
1. Beffert, U., et al. (2002) Reelin-mediated signaling locally regulates protein kinase B/Akt and glycogen synthase kinase 3beta. J. Biol. Chem. 277(51):49958-49964. 2. Hsu, J., et al. (2001) The AKT kinase is activated in multiple myeloma tumor cells. Blood 98(9):2853-2855. 3. Persad, S., et al. (2001) Regulation of protein kinase B/Akt-threonine-308 phosphorylation by integrin linked kinase (ILK): Critical roles for kinase activity and amino acids arginine-211 and threonine-343. J. Biol. Chem. 276(29):27462-27469. 4. Nelson, J.M. and D.W. Fry (2001) Akt, MAPK (Erk1/2), and p38 act in concert to promote apoptosis in response to ErbB receptor family inhibition. J. Biol. Chem. 276(18):14842-14847. 5. Genot, E.M., et al. (2000) The T-cell receptor regulates Akt (Protein Kinase B) via a pathway involving Rac1 and phosphatidylinositide 3-kinase. Mol. Cell. Biol. 20(15):5469-5478. 6. Datta, S.R., et al. (1997) Akt phosphorylation of BAD couples survival signals to the cell- intrinsic death machinery. Cell 91(2):231-241. 7. del Peso, L., et al. (1997) Interleukin-3-induced phosphorylation of BAD through the protein kinase Akt. Science 278(5338):687-689. 8. Dudek, H., et al. (1997) Regulation of neuronal survival by the serine-threonine protein kinase Akt. Science 275(5300):661-665. 9. Franke, T.F., et al. (1997) PI3K: downstream AKT phosphorylation blocks apoptosis. Cell 88(4):435-437.

Protein Reference(s)

Database Name: UniProt

Accession number: P31749


Safety Datasheet(s) for this product:
Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Mouse anti Human Akt

$508.00


Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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