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Rabbit anti Human Aif (NT) apoptosis inducing factor


Catalogue number: X1109P

Synonyms
Apoptosis Inducing Factor (NT)

IsotypeIgG
Product Type Polyclonal Antibody
Units100 µg
HostRabbit
Species reactivity Human
Application Western Blot
Product Form
Unconjugated

Background
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed (2).

Positive Control
K562 cell lysate

Immunogen
A synthetic peptide corresponding to amino acids 109 to 122 of human AIF (2). This sequence is identical to those of mouse and rat AIF (2).

Purification Method
Antigen Immunoaffiinity Purification

Concentration
See vial for concentration

Formulation
Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Functional Analysis
Western Blotting

Applications
Detects AIF by Western blot at of 0.25 to 1 µg/ml. Detects a 67 kDa band in K562 cell lysate. Optimal concentration should be evaluated by serial dilutions.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

References
1. Condensed matter in cell death. Zamzami, N. and Kroemer, G. Nature 1999, 401, 127-8 2. Molecular characterization of mitochondrial apoptosis-inducing factor. Susin, S.A., et al., Nature 1999, 397, 441-6

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide
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Rabbit anti Human Aif (NT) apoptosis inducing factor

$399.00

SKU: X1109P Categories: ,