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Rabbit anti Human Aif (NT) apoptosis inducing factor


Catalogue number: X1109P

IsotypeIgG
Product Type Polyclonal Antibody
Units100 µg
HostRabbit
Species reactivity Human
Application Western Blot

Background
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed (2).

Synonyms: Apoptosis Inducing Factor (NT)

Source

Immunogen: A synthetic peptide corresponding to amino acids 109 to 122 of human AIF (2). This sequence is identical to those of mouse and rat AIF (2).

Product

Product Form: Unconjugated

Formulation: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

Applications
Detects AIF by Western blot at of 0.25 to 1 µg/ml. Detects a 67 kDa band in K562 cell lysate. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: K562 cell lysate

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Ship Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

References
1. Condensed matter in cell death. Zamzami, N. and Kroemer, G. Nature 1999, 401, 127-8 2. Molecular characterization of mitochondrial apoptosis-inducing factor. Susin, S.A., et al., Nature 1999, 397, 441-6

Protein Reference(s)

Database Name: UniProt

Accession number: O95831 (Human) Q9Z0X1 (Mouse) Q9JM53 (Rat)

Species Accession: Human, Rat, Mouse


Safety Datasheet(s) for this product:
Sodium Azide
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Rabbit anti Human Aif (NT) apoptosis inducing factor

$407.00


Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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