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AGE-3 Glycolaldehyde modified


Catalogue number: AGE3-010

Clone9D8
IsotypeIgG1
Units10 µg
HostMouse
Application ELISA
WB
Western Blotting
Background
Glycolaldehyde-modified AGEs (AGE-3) have diverse toxic effects in living animals and humans. For example they induce apoptosis in different organs, suppress the replication rate of Schwann-cells in the rat, inhibit the differentiation of human mesenchymal stem cells. Long-term incubation of proteins with glucose leads, through Schiff's base and Amadori rearrangement products, to the formation of advanced glycation end products (AGE) which are characterized by fluorescence, brown color and inter- and intra-molecular cross-linking. Recent immunological studies using anti-AGE antibodies demonstrated the presence of AGE in (i) human lens, (ii) renal proximal tubules in patients with diabetic nephropathy and chronic renal failure, (iii) atherosclerotic lesions of arterial walls, (iv) ß2-microglobulin of carpal tunnel amyloid fibril deposits in patients with hemodialysis-related amyloidosis and (v) brain tissues of patients with Alzheimer’s disease. These results suggested the potential role of AGE in normal aging and age-enhanced disease processes. AGE-3 (Glycolaldehyde-modified proteins); in Western Blot no cross-reaction with Glucose-modified AGEs (AGE-1), AGE-2 (Glycerolaldehyde-modified), AGE-4 (Methylglyoxal-modified), AGE-5 (Glyoxal-modified), AGE-6 (3-DG-modified), CEL-BSA, CML-BSA and BSA..

Positive Control
human lens, arteriosclerotic plaques

Immunogen
Glycolaldehyde-modified BSA

Purification Method
Protein G affinity purified monoclonal antibody in PBS with 2% Block Ace as stabilizer and 0.1% Proclin as preservative

Working Concentration
(liquid conc.) 1 µg/ml (WB) and 0,1 µg/ml (ELISA)

Concentration
0.25 mg/ml

Incubation Time
60 min at RT or 18 hr at 2-8°C

Secondary Reagents
As secondary system in ELISA and Western Blot we recommend biotinylated anti-mouse IgG antibody (Art. No. ZU102) in combination with streptavidin-HRPO conjugate (Art. No. ZU054) or streptavidin-alkaline phosphatase (Art. No. ZU051).

Product
Protein G affinity purified monoclonal antibody in PBS with 2% Block Ace as stabilizer and 0.1% Proclin as preservative

Applications
ELISA, WB

Cross Reactivity
Human

Storage
-20°C

References
1. Yamagishi S., Amano S., Inagaki Y., Okamoto T., Koga K., Sasaki N., Yamamoto H., Takeuchi M., and Makita Z. (2002) Advanced Glycation End Products-Induced Apoptosis and Overexpression of Vascular Endothelial Growth Factor in Bovine Retinal Pericytes Biochemical and Biophysical Research Communications ; 290, 973-978. 2. Kume S., Kato S., Yamagishi S., Inagaki Y., Ueda S., Arima N., Okawa T., Kojiro M., Nagata K. (2005) Advanced Glycation End-Products Attenuate Human Mesenchymal Stem Cells and Prevent Cognate Differentiation Into Adipose Tissue, Cartilage, and Bone. J Bone Miner Res. 2005 Sep;20(9):1647-58. 3. Yamamoto Y., Yonekura H., Watanabe T., Sakurai S., Li H., Harashima A., Myint K.M., Osawa M., Takeuchi A., Takeuchi M., Yamamoto H. (2007) Short-chain aldehyde-derived ligands for RAGE and their actions on endothelial cells. Diabetes Res Clin Pract. 2007 Sep;77 Suppl 1:S30-40.

Caution
These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

Safety Datasheet(s) for this product:

AGE-3 Glycolaldehyde modified

$1,059.00

SKU: AGE3-010 Categories: ,