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Mouse anti Rat Adrenergic Receptor


Catalogue number: X1630M

Clone5G10
IsotypeIgG1
Product Type Monoclonal Antibody
Units100 µg
HostMouse
Species reactivity Mouse
Rat
Application Immunohistochemistry
Western Blot
Product Form
Unconjugated

Background
The rat alpha 2b adrenergic receptor is a G protein coupled receptor which acts through Gi2 and is responsible for Cellular communication and signal transduction. These receptors are linked to pertussis toxin-sensitive G proteins. Agonist binding leads to inhibition of adenylyl cyclase, inhibition of Ca(2+) channels and activation of K(+) channels. Recently, three distinct subtypes of alpha(2)-AR were described, alpha(2A)-AR, alpha(2B)-AR and alpha(2C)-AR. At present, it is unknown which of these alpha(2)-AR subtype(s) may regulate insulin secretion.

Positive Control
MDCK cells transfected with rat alpha2B adrenergic receptors.

Immunogen
Antibody raised to synthetic peptide internal sequence corresponding to the third intracellular loop of rat alpha 2b adrenergic receptor.

Purification Method
Protein A/G Chromatography

Concentration
See vial for concentration

Formulation
Provided as solution in phosphate buffered saline with 0.08% sodium azide

Functional Analysis
Western Blotting

Applications
Antibody detects alpha2B Adrenergic Receptors (1-5 µg/ml) and immunohistochemistry. Optimal concentration should be evaluated by serial dilutions.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

References
1. Ozawa, Y, et al. 'Alpha2-adrenergic receptor subtype alterations in the brainstem in the sudden infant death syndrome.' Early Hum Dev 2003, 75 Suppl, S129-38 2. Yun, J., et al. 'Gene expression profile of neurodegeneration induced by alpha1B-adrenergic receptor overactivity: NMDA/GABAA dysregulation and apoptosis.' Brain 2003, Pt 12, 2667-81 3. Peterhoff, M., et al. 'Inhibition of insulin secretion via distinct signaling pathways in alpha2-adrenoceptor knockout mice.' Eur J Endocrinol 2003, 4, 343-50 4. Paris, A., et al. 'Activation of alpha 2B-adrenoceptors mediates the cardiovascular effects of etomidate.' Anesthesiology 2003, 99, 4, 889-95 5. Brady, AE, et al. 'Spinophilin stabilizes cell surface expression of alpha 2B-adrenergic receptors.' J Biol Chem 2003, 278, 34, 32405-12 6. Savola, JM, et al. 'Fipamezole (JP-1730) is a potent alpha2 adrenergic receptor antagonist that reduces levodopa-induced dyskinesia in the MPTP-lesioned primate model of Parkinson's disease.' Mov Disord 2003, 8, 872-83 7. Sivenius, K, et al. 'A deletion in the alpha2B-adrenergic receptor gene and autonomic nervous function in central obesity.' Obes Res 2003, 8, 962-70 8. Ge, H, et al. 'Constitutive precoupling to G(i) and increased agonist potency in the alpha(2B)-adrenoceptor.' Biochem Biophys Res Commun 2003, 306, 4, 959-65 9. Sykiotis GP, et al. 'The alpha2B adrenergic receptor deletion/insertion polymorphism in morbid obesity.' Clin Auton Res 2003, 3, 203-7 10. Feldman J, Bousquet P. 'Role of cental alpha2B-adrenergic receptors in blood pressure control and hypertension.' J Hypertens 2003, 5, 871-2 11. Willems EW, et al. 'Possible role of alpha-adrenoceptor subtypes in acute migraine therapy.' Cephalalgia 2003, 4, 245-57

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Mouse anti Rat Adrenergic Receptor

$355.00

SKU: X1630M Categories: ,