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Rabbit anti Human Adiponectin


Catalogue number: X1832P

Synonyms
ACRP30, adipocyte complement-related protein 30 (NT)

IsotypeIgG
Product Type Antigen Immunoaffinity Purified Polyclonal
Units100 µg
HostRabbit
Species reactivity Human
Mouse
Rat
Application Immunohistochemistry
Western Blot
Product Form
Unconjugated

Background
Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines (reviewed in 1). One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-?-related proteins (CTRPs) 1-7 (2). Unlike the CTRPs, which are expressed in a wide variety of tissues, adiponectin is reported to be expressed exclusively by differentiated adipocytes (3). These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene (4). It is present in high levels in normal human plasma, but is reduced in obese subjects and often in those with increased insulin resistance and type 2 diabetes, suggesting that adiponectin may be a useful pharmacological target in various metabolic diseases (5).

Positive Control
Positive Control Rat brain cell lysate A secreted protein synthesized exclusively by adipocytes.6

Immunogen
Rabbit polyclonal adiponectin antibody was raised against a 15 amino acid peptide from near the amino terminus of human adiponectin.

Purification Method
Antigen Immunoaffiinity Purification

Concentration
See vial for concentration

Formulation
Provided as solution in phosphate buffered saline with 0.02% sodium azide

Functional Analysis
Western Blotting

Applications
Adiponectin antibody can be used for the detection of adiponectin by Western blot at 1 ug/ml.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

References
1) Fantuzzi G. Adipose tissue, adipokines, and inflammation. J. Allergy Clin. Immunol. 2005; 115:911-9. 2) Tsao T-S, Lodish HF, and Fruebis J. ACRP30, a new hormone controlling fat and glucose metabolism. Euro. J. Pharmacol. 2002; 440:213-21. 3.) Wong GW, Wang J, Hug C, et al. A family of Acrp30/ adiponectin structural and functional paralogs. Proc. Natl. Acad. Sci. USA 2004; 101:10302-7. 4) Shapiro L and Scherer PE. The crystal structure of a complement-1q family protein suggests an evolutionary link to tumor necrosis factor. Curr. Biol. 1998; 8:335-8. 5) Lihn AS, Pedersen SB, and Richelsen B. Adiponectin: action, regulation and association to insulin sensitivity. Obes. Rev. 2005; 6:13-21. 6) UniProtKB/Swiss-Prot entry Q15848, http://au.expasy.org/uniprot/Q15848, Accessed February 27, 2007.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Rabbit anti Human Adiponectin

$399.00


Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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