Rabbit anti Human Adiponectin
Catalogue number: X1832P
|Product Type||Antigen Immunoaffinity Purified Polyclonal|
Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines (reviewed in 1). One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-?-related proteins (CTRPs) 1-7 (2). Unlike the CTRPs, which are expressed in a wide variety of tissues, adiponectin is reported to be expressed exclusively by differentiated adipocytes (3). These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene (4). It is present in high levels in normal human plasma, but is reduced in obese subjects and often in those with increased insulin resistance and type 2 diabetes, suggesting that adiponectin may be a useful pharmacological target in various metabolic diseases (5).
Synonyms: ACRP30, adipocyte complement-related protein 30 (NT)
Immunogen: Rabbit polyclonal adiponectin antibody was raised against a 15 amino acid peptide from near the amino terminus of human adiponectin.
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.02% sodium azide
Purification Method: Antigen Immunoaffiinity Purification
Concentration: See vial for concentration
Adiponectin antibody can be used for the detection of adiponectin by Western blot at 1 ug/ml.
Functional Analysis: Western Blotting
Positive Control: Positive Control Rat brain cell lysate A secreted protein synthesized exclusively by adipocytes.6
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Ship Conditions: Ship at ambient temperature, freeze upon arrival
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
1) Fantuzzi G. Adipose tissue, adipokines, and inflammation. J. Allergy Clin. Immunol. 2005; 115:911-9. 2) Tsao T-S, Lodish HF, and Fruebis J. ACRP30, a new hormone controlling fat and glucose metabolism. Euro. J. Pharmacol. 2002; 440:213-21. 3.) Wong GW, Wang J, Hug C, et al. A family of Acrp30/ adiponectin structural and functional paralogs. Proc. Natl. Acad. Sci. USA 2004; 101:10302-7. 4) Shapiro L and Scherer PE. The crystal structure of a complement-1q family protein suggests an evolutionary link to tumor necrosis factor. Curr. Biol. 1998; 8:335-8. 5) Lihn AS, Pedersen SB, and Richelsen B. Adiponectin: action, regulation and association to insulin sensitivity. Obes. Rev. 2005; 6:13-21. 6) UniProtKB/Swiss-Prot entry Q15848, http://au.expasy.org/uniprot/Q15848, Accessed February 27, 2007.
Database Name: UniProt
Accession number: Q15848
Species Accession: Human
Safety Datasheet(s) for this product:
Rabbit anti Human Adiponectin